EIF2AK1 Antibody (1G4Z2)

Western Blot: EIF2AK1 Antibody (1G4Z2) [NBP3-33479] - Western Blot analysis of lysates from wild type(WT) and EIF2AK1 knockdown (KD) 293T cells, using [KD Validated] EIF2AK1 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1G4Z2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

EIF2AK1 Antibody (1G4Z2) Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 337-586 of human EIF2AK1.2).
  
  Sequence:
  SIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNS
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF2AK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:1000 - 1:5000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for EIF2AK1 Antibody (1G4Z2)

• EC 2.7.11
• eukaryotic translation initiation factor 2-alpha kinase 1
• HCR
• heme sensitive initiation factor 2a kinase
• Heme-controlled repressor
• Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase
• Heme-regulated inhibitor
• heme-regulated initiation factor 2-alpha kinase
• heme-regulated repressor
• Hemin-sensitive initiation factor 2-alpha kinase
• HRIEC 2.7.11.1
• KIAA1369heme regulated initiation factor 2 alpha kinase

Background

EIF2AK1 is encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF2AK1