| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83210-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1980-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77266-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37242-0.025ml | |||
![Western Blot: EIF2AK1 Antibody [NBP1-83210] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)](http://images.novusbio.com/fullsize/EIF2AK1-Antibody-Western-Blot-NBP1-83210-img0005.jpg "Western Blot: EIF2AK1 Antibody [NBP1-83210] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)")
![Immunocytochemistry/Immunofluorescence: EIF2AK1 Antibody [NBP1-83210] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EIF2AK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83210-img0006.jpg)
![Immunohistochemistry-Paraffin: EIF2AK1 Antibody [NBP1-83210] - Staining of human corpus, uterine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EIF2AK1-Antibody-Immunohistochemistry-Paraffin-NBP1-83210-img0002.jpg)
![Western Blot: EIF2AK1 Antibody [NBP1-56484] - Human Hela, Antibody Dilution: 1.0 ug/ml EIF2AK1 is strongly supported by BioGPS gene expression data to be expressed in HeLa.](http://images.novusbio.com/fullsize/EIF2AK1-Antibody-Western-Blot-NBP1-56484-img0003.jpg "Western Blot: EIF2AK1 Antibody [NBP1-56484] - Human Hela, Antibody Dilution: 1.0 ug/ml EIF2AK1 is strongly supported by BioGPS gene expression data to be expressed in HeLa.")
![Immunohistochemistry-Paraffin: EIF2AK1 Antibody [NBP1-56484] - Human Lung tissue, 5ug/ml.](http://images.novusbio.com/fullsize/EIF2AK1-Antibody-Immunohistochemistry-Paraffin-NBP1-56484-img0002.jpg)
![Western Blot: EIF2AK1 Antibody [NBP1-56484] - Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Human Lung](http://images.novusbio.com/fullsize/EIF2AK1-Antibody-Western-Blot-NBP1-56484-img0001.jpg)
![Western Blot: EIF2AK1 Antibody (1G4Z2) [NBP3-33479] - Western Blot analysis of lysates from wild type(WT) and EIF2AK1 knockdown (KD) 293T cells, using [KD Validated] EIF2AK1 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-33479_rabbit-eif2ak1-mab-1g4z2-31120251704794.jpg "<b>Genetic Strategies Validation. </b>Western Blot: EIF2AK1 Antibody (1G4Z2) [NBP3-33479] - Western Blot analysis of lysates from wild type(WT) and EIF2AK1 knockdown (KD) 293T cells, using [KD Validated] EIF2AK1 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Immunoprecipitation:[NBP3-29375]-Detection of human EIF2AK1 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-EIF2AK1 antibody used for IP at 10 µg/mg lysate. EIF2AK1 was also immunoprecipitated by rabbit anti-EIF2AK1 antibody , which recognizes a downstream epitope. For blotting immunoprecipitated EIF2AK1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29375_rabbit-eif2ak1-pab-231202512101617.jpg "Immunoprecipitation:[NBP3-29375]-Detection of human EIF2AK1 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-EIF2AK1 antibody used for IP at 10 µg/mg lysate. EIF2AK1 was also immunoprecipitated by rabbit anti-EIF2AK1 antibody , which recognizes a downstream epitope. For blotting immunoprecipitated EIF2AK1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:EIF2AK1 AntibodyNBP3-29399] -Detection of human EIF2AK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EIF2AK1 antibody NBP3-29399 used for IP at 10 µg/mg lysate. EIF2AK1 was also immunoprecipitated by rabbit anti-EIF2AK1., which recognizes an upstream epitope. For blotting immunoprecipitated EIF2AK1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29399_rabbit-eif2ak1-pab-301220241652448.jpg "Immunoprecipitation:EIF2AK1 AntibodyNBP3-29399] -Detection of human EIF2AK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EIF2AK1 antibody NBP3-29399 used for IP at 10 µg/mg lysate. EIF2AK1 was also immunoprecipitated by rabbit anti-EIF2AK1., which recognizes an upstream epitope. For blotting immunoprecipitated EIF2AK1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/ Immunofluorescence: PKR Antibody - BSA Free [NBP1-77266] - Validation of PKR in Mouse Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77266_rabbit-polyclonal-pkr-antibody-271120242092073.gif "Immunocytochemistry/ Immunofluorescence: PKR Antibody - BSA Free [NBP1-77266] - Validation of PKR in Mouse Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).")
![Western Blot: PKR Antibody [NBP1-77266] - WB Validation of PKR in Human Cell Lines. Antibodies: PKR. Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/PKR-Antibody-Western-Blot-NBP1-77266-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PKR Antibody [NBP1-77266] - IAV via Protein Expression Profile in Cell Lines. Antibodies PKR and beta-actin. Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/PKR-Antibody-Western-Blot-NBP1-77266-img0008.jpg)
![Immunohistochemistry: PKR Antibody - BSA Free [NBP1-77266] - Validation of PKR in Mouse Lung. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-PKR antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77266_rabbit-polyclonal-pkr-antibody-2711202420195341.gif)
![Immunocytochemistry/ Immunofluorescence: PKR Antibody - BSA Free [NBP1-77266] - Validation of PKR in Mouse LungImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77266_rabbit-polyclonal-pkr-antibody-271120242013150.jpg)
![Immunohistochemistry: PKR Antibody - BSA Free [NBP1-77266] - Validation of PKR in Rat Lung. Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-PKR antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77266_rabbit-polyclonal-pkr-antibody-271120242016933.jpg)
![Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Analysis using PKR mAb against (1) HEK293 and (2) PKR (AA: 329-551)-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/PKR-Antibody-6H3A10-Western-Blot-NBP2-37242-img0010.jpg "Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Analysis using PKR mAb against (1) HEK293 and (2) PKR (AA: 329-551)-hIgGFc transfected HEK293 cell lysate.")
![Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of cervical cancer tissues using EIF2AK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PKR-Antibody-6H3A10-Immunohistochemistry-Paraffin-NBP2-37242-img0007.jpg)
![Flow Cytometry: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of A431 cells using EIF2AK2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PKR-Antibody-6H3A10-Flow-Cytometry-NBP2-37242-img0004.jpg)
![Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Western blot analysis using EIF2AK2 mAb against human EIF2AK2 (AA: 329-551) recombinant protein. (Expected MW is 51.7 kDa)](http://images.novusbio.com/fullsize/PKR-Antibody-6H3A10-ELISA-NBP2-37242-img0002.jpg)
![Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Analysis using EIF2AK2 mouse mAb against A431 (1), THP-1 (2), MCF-7 (3), PC-12 (4) cell lysate.](http://images.novusbio.com/fullsize/PKR-Antibody-6H3A10-Western-Blot-NBP2-37242-img0003.jpg)
![Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of rectum cancer tissues using EIF2AK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PKR-Antibody-6H3A10-Immunohistochemistry-Paraffin-NBP2-37242-img0008.jpg)
![ELISA: PKR Antibody (6H3A10) [NBP2-37242] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/PKR-Antibody-6H3A10-ELISA-NBP2-37242-img0001.jpg)
![Western Blot: PKR [p Thr446] Antibody (SY230) [NBP2-67426] - Analysis of Phospho-PKR(T446) on different lysates using anti-Phospho-PKR(T446) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela treated with Calyculin A and TNF-alpha whole cell lysates Lane 2: Untreated Hela whole cell lysates](http://images.novusbio.com/fullsize/PKR-p-Thr446-Antibody-SY230-Western-Blot-NBP2-67426-img0002.jpg "Western Blot: PKR [p Thr446] Antibody (SY230) [NBP2-67426] - Analysis of Phospho-PKR(T446) on different lysates using anti-Phospho-PKR(T446) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela treated with Calyculin A and TNF-alpha whole cell lysates Lane 2: Untreated Hela whole cell lysates")
![Immunohistochemistry-Paraffin: PKR [p Thr446] Antibody (SY230) [NBP2-67426] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/PKR-p-Thr446-Antibody-SY230-Immunohistochemistry-Paraffin-NBP2-67426-img0008.jpg)
![Immunohistochemistry-Paraffin: PKR [p Thr446] Antibody (SY230) [NBP2-67426] - Analysis of paraffin-embedded human kidney tissue using anti-Phospho-PKR(T446) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PKR-p-Thr446-Antibody-SY230-Immunohistochemistry-Paraffin-NBP2-67426-img0001.jpg)
![Immunohistochemistry-Paraffin: PKR [p Thr446] Antibody (SY230) [NBP2-67426] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PKR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/PKR-p-Thr446-Antibody-SY230-Immunohistochemistry-Paraffin-NBP2-67426-img0003.jpg)
![Immunohistochemistry-Paraffin: PKR [p Thr446] Antibody (SY230) [NBP2-67426] - Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PKR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/PKR-p-Thr446-Antibody-SY230-Immunohistochemistry-Paraffin-NBP2-67426-img0004.jpg)
![Immunohistochemistry-Paraffin: PKR [p Thr446] Antibody (SY230) [NBP2-67426] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/PKR-p-Thr446-Antibody-SY230-Immunohistochemistry-Paraffin-NBP2-67426-img0005.jpg)
![Immunohistochemistry-Paraffin: PKR [p Thr446] Antibody (SY230) [NBP2-67426] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-PKR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/PKR-p-Thr446-Antibody-SY230-Immunohistochemistry-Paraffin-NBP2-67426-img0006.jpg)
![Immunohistochemistry-Paraffin: PKR [p Thr446] Antibody (SY230) [NBP2-67426] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/PKR-p-Thr446-Antibody-SY230-Immunohistochemistry-Paraffin-NBP2-67426-img0007.jpg)
![Western Blot: PKR Antibody (1D11) [H00005610-M02] - Analysis of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M02), clone 1D11.Lane 1: EIF2AK2 transfected lysate(62.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PKR-Antibody-1D11-Western-Blot-H00005610-M02-img0012.jpg "Western Blot: PKR Antibody (1D11) [H00005610-M02] - Analysis of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M02), clone 1D11.Lane 1: EIF2AK2 transfected lysate(62.1 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PKR Antibody (1D11) [H00005610-M02] - Analysis of monoclonal antibody to EIF2AK2 on HeLa cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/fullsize/PKR-Antibody-1D11-Immunocytochemistry-Immunofluorescence-H00005610-M02-img0007.jpg)
![Immunohistochemistry-Paraffin: PKR Antibody (1D11) [H00005610-M02] - Analysis of monoclonal antibody to EIF2AK2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/PKR-Antibody-1D11-Immunohistochemistry-Paraffin-H00005610-M02-img0009.jpg)
![Western Blot: PKR Antibody (1D11) [H00005610-M02] - EIF2AK2 monoclonal antibody (M02), clone 1D11 Analysis of EIF2AK2 expression in K-562.](http://images.novusbio.com/fullsize/PKR-Antibody-1D11-Western-Blot-H00005610-M02-img0011.jpg)
![ELISA: PKR Antibody (1D11) [H00005610-M02] - Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PKR-Antibody-1D11-ELISA-H00005610-M02-img0013.jpg)
![Western Blot: PKR [p Thr446] Antibody [NB100-82156] - Analysis of extracts from K562 cells using PKR(Phospho-Thr446) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).](http://images.novusbio.com/fullsize/PKR-[p-Thr446]-Antibody-Western-Blot-NB100-82156-img0002.jpg "Western Blot: PKR [p Thr446] Antibody [NB100-82156] - Analysis of extracts from K562 cells using PKR(Phospho-Thr446) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).")
![Western Blot: PKR [p Thr449] Antibody (2F8) [NBP3-15204] - Positive WB detected in Hela whole cell lysate (treated with Calyculin A or not). All lanes PKR antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 KDa. Observed band size: 62 KDa.](http://images.novusbio.com/fullsize/PKR-p-Thr449-Antibody-2F8-Western-Blot-NBP3-15204-img0003.jpg "Western Blot: PKR [p Thr449] Antibody (2F8) [NBP3-15204] - Positive WB detected in Hela whole cell lysate (treated with Calyculin A or not). All lanes PKR antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 KDa. Observed band size: 62 KDa.")
![Immunocytochemistry/Immunofluorescence: PKR [p Thr449] Antibody (2F8) [NBP3-15204] - Immunofluorescence staining of Hela cells (treated with 50mM Calyculin A for 30 minutes) with at PKR antibody 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/PKR-p-Thr449-Antibody-2F8-Immunocytochemistry-Immunofluorescence-NBP3-15204-img0001.jpg)
![Immunohistochemistry: PKR [p Thr449] Antibody (2F8) [NBP3-15204] - IHC image of PKR antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/PKR-p-Thr449-Antibody-2F8-Immunohistochemistry-NBP3-15204-img0002.jpg)