eIF2 alpha/EIF2S1 Antibody (OTI3H7)

Product Discontinued

eIF2 alpha/EIF2S1 Antibody (OTI3H7) Summary

• Immunogen: Full length recombinant protein of human eIF2 alpha/EIF2S1 (NP_004085) produced in HEK293T cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF2S1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Western Blot 1:1000-2000
• Theoretical MW: 35.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Dog, Rat, Monkey

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for eIF2 alpha/EIF2S1 Antibody (OTI3H7)

• eIF2 alpha
• EIF2
• EIF-2
• EIF2A
• EIF-2A
• EIF-2alpha
• EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• EIF2S1

Background

EIF2S1, also known as eIF2a, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF2S1
• Entrez:
  • 1965(Human)