eIF2 alpha/EIF2S1 Antibody (5E10) - BSA Free

Western Blot: eIF2 alpha/EIF2S1 Antibody (5E10) [NBP1-97936] - The extracts of HeLa (50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (5E10) [NBP1-97936] - Analysis of EIF2S1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human EIF2S1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western Blot: eIF2 alpha/EIF2S1 Antibody (5E10) [NBP1-97936] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: Jurkat cell lysate Lane 3.: NIH-3T3 cell lysate

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 5E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

eIF2 alpha/EIF2S1 Antibody (5E10) - BSA Free Summary

• Description: Novus Biologicals Mouse eIF2 alpha/EIF2S1 Antibody (5E10) - BSA Free (NBP1-97936) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human eIF2 alpha/EIF2S1 (1-315aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF2S1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for eIF2 alpha/EIF2S1 Antibody (5E10) - BSA Free

• eIF2 alpha
• EIF2
• EIF-2
• EIF2A
• EIF-2A
• EIF-2alpha
• EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• EIF2S1

Background

EIF2S1, also known as eIF2a, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF2S1
• Uniprot:
  • NP_004085(Human)