EIF1A Antibody (7G7Y3)

Western Blot: EIF1A Antibody (7G7Y3) [NBP3-33249] - Western blot analysis of various lysates, using EIF1A Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 7G7Y3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

EIF1A Antibody (7G7Y3) Summary

• Description: Novus Biologicals Rabbit EIF1A Antibody (7G7Y3) (NBP3-33249) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 65-144 of human EIF1A (P47813).
  
  Sequence:
  LRKKVWINTSDIILVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDTFGPGDDDEIQFDDIGDDDEDIDDI
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF1AX
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for EIF1A Antibody (7G7Y3)

• eIF-1A
• EIF1AP1
• EIF4C
• eIF-4C
• eukaryotic translation initiation factor 1A, X chromosome
• eukaryotic translation initiation factor 1A, X-chromosomal
• eukaryotic translation initiation factor 1A, Y chromosome
• eukaryotic translation initiation factor 1A, Y-chromosomal
• Eukaryotic translation initiation factor 4C
• FLJ45762
• Putative eukaryotic translation initiation factor 1A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF1AX