EHF Antibody

Western Blot: EHF Antibody [NBP2-14942] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: EHF Antibody [NBP2-14942] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: EHF Antibody [NBP2-14942] - Paraffin-embedded Muscle, using antibody at 1:500 dilution.

Product Discontinued

EHF Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human EHF. The exact sequence is proprietary.
• Predicted Species: Rat (92%), Bovine (93%), Chimpanzee (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EHF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for EHF Antibody

• Epithelium-specific Ets transcription factor 3
• ESE3 transcription factor
• ESE-3
• ESE3B
• ESE3hEHF
• ESEJepithelium-specific ets factor 3
• ETS domain-containing transcription factor
• ets homologous factor

Background

This gene encodes a protein that belongs to an ETS transcription factor subfamily characterized by epithelial-specific expression (ESEs). The encoded protein acts as a transcriptional repressor and may be associated with asthma susceptibility. This protein may be involved in epithelial differentiation and carcinogenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for EHF Antibody (NBP2-14942)(1)

Publication using NBP2-14942

• Li J, Yang J, Hua L et al. Ese-3 contributes to colon cancer progression by downregulating EHD2 and transactivating INPP4B American journal of cancer research 2021-01-01 [PMID: 33520362] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: EHF
• Uniprot:
  • Q9NZC4(Human)