Recombinant Human EG-VEGF/PK1 Animal-Free Protein

SDS-Page: Recombinant Human EG-VEGF/PK1 Animal-Free Protein [NBP2-76239] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Product Discontinued

Recombinant Human EG-VEGF/PK1 Animal-Free Protein Summary

• Description: For prolonged storage, dilute to working aliquots.
  
  Expiration Date:
  12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
• Specificity: EG-VEGF/PK1 is a monomer, 9.7 kDa (86 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PROK1
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Lot Specific Information:
  Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
• Theoretical MW: 9.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Alternate Names for Recombinant Human EG-VEGF/PK1 Animal-Free Protein

• EGVEGF
• EG-VEGF
• EGVEGFblack mamba toxin-related protein
• Endocrine-gland-derived vascular endothelial growth factor
• Mambakine
• PK1mambakine
• PRK1prokineticin-1
• PROK1
• Prokineticin 1

Background

Prokineticin 1 potently contracts gastrointestinal (GI) smooth muscle. It induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands and has little or no effect on a variety of other endothelial and non-endothelial cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PROK1