EG-VEGF/PK1 Overexpression Lysate (Native)

Images

 
Western Blot: Prokineticin 1 Overexpression Lysate (Adult Normal) [NBL1-14806] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Prokineticin 1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EG-VEGF/PK1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205595 and based on accession number NM_032414. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens prokineticin 1 (PROK1), mRNA.
Gene
PROK1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
11.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for EG-VEGF/PK1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EG-VEGF/PK1 Overexpression Lysate (Native)

  • EGVEGF
  • EG-VEGF
  • EGVEGFblack mamba toxin-related protein
  • Endocrine-gland-derived vascular endothelial growth factor
  • Mambakine
  • PK1mambakine
  • PRK1prokineticin-1
  • PROK1
  • Prokineticin 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-43853
Species: Hu, Mu, Rt, Bv
Applications: WB, ELISA, ICC/IF, IHC-Fr, IHC-P
MAB4655
Species: Hu
Applications: Flow, CyTOF-ready
NBP1-92290
Species: Hu
Applications: IHC, IHC-P
NBP1-48308
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-47935
Species: Hu, Mk
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02520
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-57614
Species: Hu
Applications: ICC/IF
NBP1-90367
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-56159
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-P, IP
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-37573
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, IHC
NB100-1617
Species: Hu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB100-91273
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBL1-14806
Species: Hu
Applications: WB

Publications for EG-VEGF/PK1 Lysate (NBL1-14806) (0)

There are no publications for EG-VEGF/PK1 Lysate (NBL1-14806).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EG-VEGF/PK1 Lysate (NBL1-14806) (0)

There are no reviews for EG-VEGF/PK1 Lysate (NBL1-14806). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EG-VEGF/PK1 Lysate (NBL1-14806) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional EG-VEGF/PK1 Products

Bioinformatics Tool for EG-VEGF/PK1 Lysate (NBL1-14806)

Discover related pathways, diseases and genes to EG-VEGF/PK1 Lysate (NBL1-14806). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EG-VEGF/PK1 Lysate (NBL1-14806)

Discover more about diseases related to EG-VEGF/PK1 Lysate (NBL1-14806).
 

Pathways for EG-VEGF/PK1 Lysate (NBL1-14806)

View related products by pathway.

PTMs for EG-VEGF/PK1 Lysate (NBL1-14806)

Learn more about PTMs related to EG-VEGF/PK1 Lysate (NBL1-14806).
 

Research Areas for EG-VEGF/PK1 Lysate (NBL1-14806)

Find related products by research area.

Blogs on EG-VEGF/PK1

There are no specific blogs for EG-VEGF/PK1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EG-VEGF/PK1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol PROK1
Entrez