Human EG-VEGF/PK1 ELISA Kit (Colorimetric) Summary
| Description |
PROK1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human PROK1. |
| Standard Curve Range |
15.6 to 1000 pg/mL |
| Sensitivity |
<7 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 6.6% |
| Intra-Assay |
CV% < 4.8% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
PROK1 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Kit Components
|
Components
|
- 96-well Plate precoated with anti- human EG-VEGF antibody
- ABC diluent buffer
- Adhesive cover
- Antibody diluent buffer
- Avidin-Biotin-Peroxidase Complex (ABC) (dilution 1:100)
- Biotinylated anti- human EG-VEGF antibody (dilution 1:100)
- Lyophilized recombinant human EG-VEGF standard
- Sample diluent buffer
- TMB color developing agent
- TMB stop solution
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human EG-VEGF/PK1 ELISA Kit (Colorimetric)
Background
Prokineticin 1 potently contracts gastrointestinal (GI) smooth muscle. It induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands and has little or no effect on a variety of other endothelial and non-endothelial cell types.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for EG-VEGF/PK1 ELISA Kit (KA1675). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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