Recombinant Human EG-VEGF/PK1 Protein

• Reactivity: Hu
• Applications: Bioactivity
• Concentration: LYOPH

Recombinant Human EG-VEGF/PK1 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 86 amino acids corresponding to EG-VEGF/PK1 Source: E. coli

  Uniprot ID: P58294

  Amino Acid Sequence: AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF

• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using bovine EJG cells is less than 2.0 ug/ml, corresponding to a specific activity of > 500 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PROK1
• Purity: >98%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of EG-VEGF/PK1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 9.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 0.02 % Tween-20.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >98%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human EG-VEGF/PK1 Protein

• EGVEGF
• EG-VEGF
• EGVEGFblack mamba toxin-related protein
• Endocrine-gland-derived vascular endothelial growth factor
• Mambakine
• PK1mambakine
• PRK1prokineticin-1
• PROK1
• Prokineticin 1

Background

Prokineticin 1 potently contracts gastrointestinal (GI) smooth muscle. It induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands and has little or no effect on a variety of other endothelial and non-endothelial cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PROK1