EFTUD2 Antibody (1-D10-A1-R)

Western Blot: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Western blot analysis of EFTUD2 on different lysates with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HEK-293 cell lysate Lane 4: U-87 MG cell lysate Lane 5: LNCaP cell lysate Lane 6: HepG2 cell lysate Lane 7: K-562 cell lysate Lane 8: C6 cell lysate Lane 9: PC-12 cell lysate Lane 10: NIH/3T3 cell lysate Lane 11: RAW264.7 cell lysate Lane 12: Mouse testis tissue lysate Lane 13: Mouse kidney tissue lysate Lane 14: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 109 kDa Observed band size: 120 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32295) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Immunofluorescence analysis of paraffin-embedded human breast cancer tissue labeling EFTUD2 with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32295, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32295) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Immunocytochemistry analysis of HeLa cells labeling EFTUD2 with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Flow cytometric analysis of HeLa cells labeling EFTUD2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32295, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: 1-D10-A1-R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EFTUD2 Antibody (1-D10-A1-R) Summary

• Description: Novus Biologicals Mouse EFTUD2 Antibody (1-D10-A1-R) (NBP3-32295) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within mouse EFTUD2 aa 1-50 / 972. (Uniprot: Q15029)
• Localization: Nucleus.
• Source: HEK293
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EFTUD2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:20000
  • Western Blot 1:2000
• Theoretical MW: 109 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for EFTUD2 Antibody (1-D10-A1-R)

• DKFZp686E24196
• elongation factor Tu GTP binding domain containing 2
• Elongation factor Tu GTP-binding domain-containing protein 2
• FLJ44695
• hSNU114
• KIAA0031U5-116 kDa
• SNRP116
• Snrp116,116 kDa U5 small nuclear ribonucleoprotein component
• SNU114 homolog
• Snu114
• U5 snRNP specific protein, 116 kD
• U5 snRNP-specific protein, 116 kD
• U5 snRNP-specific protein, 116 kDa
• U5-116KD

Background

EFTUD2 is one of several proteins that make up the U5 small nuclear ribonucleoprotein (snRNP) particle, which is a component of the spliceosome. It contains consensus GTPase motifs, and it has been shown that this protein binds GTP specifically.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EFTUD2