EFTUD2 Antibodies
We offer EFTUD2 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each EFTUD2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our EFTUD2 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the EFTUD2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our EFTUD2 polyclonal antibodies.
Alternate Names for EFTUD2 Antibodies
anti-EFTUD2 antibody, anti-DKFZp686E24196 antibody, anti-elongation factor Tu GTP binding domain containing 2 antibody, anti-Elongation factor Tu GTP-binding domain-containing protein 2 antibody, anti-FLJ44695 antibody, anti-hSNU114 antibody, anti-KIAA0031U5-116 kDa antibody, anti-SNRP116 antibody, anti-Snrp116,116 kDa U5 small nuclear ribonucleoprotein component antibody, anti-Snu114 antibody, anti-SNU114 homolog antibody, anti-U5 snRNP specific protein, 116 kD antibody, anti-U5 snRNP-specific protein, 116 kD antibody, anti-U5 snRNP-specific protein, 116 kDa antibody, anti-U5-116KD antibody
6 Results for "eftud2" in Primary Antibodies
![Western Blot: EFTUD2 Antibody [NB100-40849] - a The protein and mRNA levels of EFTUD2 expression in HCC cells detected by western blot and qPCR. b Western blot analysis of EFTUD2 expression in Hep3B and Huh7 cells infected with siRNA against EFTUD2 (siEFTUD2). e Western blot analysis of EFTUD2 protein expression in Hep3B and Huh7 cells infected with lentiviruses expressing either scrambled shRNA or shRNA against EFTUD2 (shEFTUD2). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-03040-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Knockdown-Validated-NB100-40849-img0006.jpg "<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: EFTUD2 Antibody [NB100-40849] - a The protein and mRNA levels of EFTUD2 expression in HCC cells detected by western blot and qPCR. b Western blot analysis of EFTUD2 expression in Hep3B and Huh7 cells infected with siRNA against EFTUD2 (siEFTUD2). e Western blot analysis of EFTUD2 protein expression in Hep3B and Huh7 cells infected with lentiviruses expressing either scrambled shRNA or shRNA against EFTUD2 (shEFTUD2). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-03040-5) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry: EFTUD2 Antibody [NB100-40849] - Serial sections of human HCC tissue samples were subjected to IHC staining with antibodies against EFTUD2. Representative IHC images from 50 human HCC tissue samples. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-03040-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunohistochemistry-NB100-40849-img0007.jpg)
![Immunohistochemistry-Paraffin: EFTUD2 Antibody [NB100-40849] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-EFTUD2/SNRP116 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunohistochemistry-Paraffin-NB100-40849-img0005.jpg)
![Immunoprecipitation: EFTUD2 Antibody [NB100-40849] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Affinity purified rabbit anti- EFTUD2/SNRP116 antibody 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate (B).](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunoprecipitation-NB100-40849-img0003.jpg)
![Western Blot: EFTUD2 Antibody [NB100-40849] - EFTUD2 expression is necessary for HCC cell survival.a The protein & mRNA levels of EFTUD2 expression in HCC cells detected by western blot & qPCR. b Western blot analysis of EFTUD2 expression in Hep3B & Huh7 cells infected with siRNA against EFTUD2 (siEFTUD2). c. d The effect of EFTUD2 on cellular viability was detected by CCK8 assays & colony formation assays, respectively. e Western blot analysis of EFTUD2 protein expression in Hep3B & Huh7 cells infected with lentiviruses expressing either scrambled shRNA or shRNA against EFTUD2 (shEFTUD2). f Representative images show the cell morphological changes after the stable knockdown of EFTUD2. Scale bars, 100 μm. g, h CCK8 assays & colony formation assays show the effect of EFTUD2 on cellular survival in Hep3B & Huh7 cells with a stable knockdown of EFTUD2. The data are presented as means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33024090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-40849_rabbit-polyclonal-eftud2-antibody-310202415541961.jpg)
![Western Blot: EFTUD2 Antibody [NB100-40849] - EFTUD2 promotes EMT via activation of the STAT3 pathway in HCC cells.a An M–A plot showed the genes differentially expressed between EFTUD2-overexpressing & control Hep G2 cells (N = 3), with the differentially regulated genes highlighted in red. b Gene sets of the IL6_JAK_STAT3 signaling pathway & the EMT were enriched in EFTUD2-overexpressing Hep G2 cells. c Serial sections of the xenograft tumors were subjected to IHC staining of EFTUD2, pSTAT3, E-cadherin & vimentin. Scale bars, 100 μm. d Serial sections of human HCC tissue samples were subjected to IHC staining with antibodies against EFTUD2 & pSTAT3. Representative IHC images were showed in the left panel, & the right panel showed the correlation between EFTUD2 & pSTAT3 expression, in 50 human HCC tissue samples. Scale bars, 100 μm. e The expression of EFTUD2, vimentin, Twist1, E-cadherin, STAT3, pSTAT3, MCL-1, & GAPDH was detected by western blotting in the indicated cells. f Western blot analysis showing that the increases in pSTAT3 & vimentin expression were compromised & that the decrease in E-cadherin was reversed by SI3-201, a STAT3 inhibitor, in EFTUD2-overexpressing Hep G2 cells. g The Hep G2 cells with EFTUD2 overexpression were treated with 100 μM SI3-201 & subjected to migration & invasion assays. Scale bars, 200 μm. The data are presented as the mean ± SD from triplicate independent experiments, **P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33024090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-40849_rabbit-polyclonal-eftud2-antibody-310202415525297.jpg)
![Western Blot: EFTUD2 Antibody [NB100-40849] - EFTUD2 promotes EMT via activation of the STAT3 pathway in HCC cells.a An M–A plot showed the genes differentially expressed between EFTUD2-overexpressing & control Hep G2 cells (N = 3), with the differentially regulated genes highlighted in red. b Gene sets of the IL6_JAK_STAT3 signaling pathway & the EMT were enriched in EFTUD2-overexpressing Hep G2 cells. c Serial sections of the xenograft tumors were subjected to IHC staining of EFTUD2, pSTAT3, E-cadherin & vimentin. Scale bars, 100 μm. d Serial sections of human HCC tissue samples were subjected to IHC staining with antibodies against EFTUD2 & pSTAT3. Representative IHC images were showed in the left panel, & the right panel showed the correlation between EFTUD2 & pSTAT3 expression, in 50 human HCC tissue samples. Scale bars, 100 μm. e The expression of EFTUD2, vimentin, Twist1, E-cadherin, STAT3, pSTAT3, MCL-1, & GAPDH was detected by western blotting in the indicated cells. f Western blot analysis showing that the increases in pSTAT3 & vimentin expression were compromised & that the decrease in E-cadherin was reversed by SI3-201, a STAT3 inhibitor, in EFTUD2-overexpressing Hep G2 cells. g The Hep G2 cells with EFTUD2 overexpression were treated with 100 μM SI3-201 & subjected to migration & invasion assays. Scale bars, 200 μm. The data are presented as the mean ± SD from triplicate independent experiments, **P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33024090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-40849_rabbit-polyclonal-eftud2-antibody-31020241683938.jpg)
![Western Blot: EFTUD2 Antibody [NB100-40849] - Depletion of EFTUD2 blocks cell cycle progression & promotes cell apoptosis.a EdU incorporation assays were used to identify the cells in S phase after EFTUD2 silencing in the indicated cells. Red: cell in S phase. Scale bars, 200 μm. b The cell cycle analyses were performed in Hep3B & Huh7 cells with EFTUD2 knockdown. c, d Fluorescence microscopy & flow cytometry were performed to captured early apoptotic cells by mitochondrial membrane potential changes in Hep3B & Huh7 cells with EFTUD2 knockdown. Red, JC-1 aggregates; green, JC-1 monomers. Scale bars, 10 μm. e, f EFTUD2-silenced Hep3B & Huh7 cells treated with or without CDDP (2 μg/mL; 48 h) were stained with a combination of Annexin V-FITC & PI & analyzed by FACS. Cells positive for FITC staining were counted as apoptotic cells. The bar graphs show the percentage of apoptotic cells. g Apoptosis-associated proteins of the indicated cells were measured by western blotting. The data are presented as the means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33024090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-40849_rabbit-polyclonal-eftud2-antibody-310202415533811.jpg)
![Western Blot: EFTUD2 Antibody [NB100-40849] - EFTUD2 promotes HCC growth.a Western blot analysis of EFTUD2 protein expression in Hep G2 cells infected with the EFTUD2 overexpression lentivirus or the vector lentivirus. b, c The effect of EFTUD2 overexpression on cell proliferation was detected by CCK8 assays & colony formation assays. d EdU incorporation assays were used to identify the cells in S phase. e The distribution of different cell cycle phases in the indicated cells. f Tumor volumes were measured & recorded every 3 days, & a growth curve was plotted. g Subcutaneous xenograft tumors of the indicated cells. The right panel shows the wet weights of the xenograft tumors. h Representative images of H&E & IHC staining of EFTUD2 & Ki67 in the xenograft tumors. Scale bars, 100 μm. The data are presented as the means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; NS no significance. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33024090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-40849_rabbit-polyclonal-eftud2-antibody-31020241554528.jpg)
![Immunocytochemistry/Immunofluorescence: EFTUD2 Antibody [NBP2-92930] - Analysis of C6 cells using EFTUD2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92930-img0014.jpg "Immunocytochemistry/Immunofluorescence: EFTUD2 Antibody [NBP2-92930] - Analysis of C6 cells using EFTUD2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: EFTUD2 Antibody [NBP2-92930] - Analysis of HeLa cells using EFTUD2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92930-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: EFTUD2 Antibody [NBP2-92930] - Analysis of L929 cells using EFTUD2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92930-img0002.jpg)
![Immunohistochemistry-Paraffin: EFTUD2 Antibody [NBP2-92930] - Analysis of EFTUD2 in paraffin-embedded rat brain tissue using EFTUD2 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-92930_rabbit-polyclonal-eftud2-antibody-immunohistochemistry-paraffin-267202491347..jpg)
![Immunohistochemistry-Paraffin: EFTUD2 Antibody [NBP2-92930] - Analysis of EFTUD2 in mouse brain tissue using EFTUD2 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp2-92930_rabbit-polyclonal-eftud2-antibody-immunohistochemistry-paraffin-267202491455..jpg)
![Immunoprecipitation: EFTUD2 Antibody - BSA Free [NBP2-92930] - Immunoprecipitation analysis of 300 ug extracts of NTH-3T3 cells using 3 ug EFTUD2 Rabbit pAb . Western blot was performed from the immunoprecipitate using EFTUD2 Rabbit pAb at a dilition of 1:2000.](http://images.novusbio.com/fullsize/nbp2-92930_rabbit-polyclonal-eftud2-antibody-4220251429032.jpg%3Ft=1705994986)
![Western Blot: EFTUD2 Antibody - BSA Free [NBP2-92930] - Western blot analysis of various lysates, using EFTUD2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92930_rabbit-polyclonal-eftud2-antibody-4220251430478.jpg%3Ft=1705994986)
![Western Blot: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Western blot analysis of EFTUD2 on different lysates with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HEK-293 cell lysate Lane 4: U-87 MG cell lysate Lane 5: LNCaP cell lysate Lane 6: HepG2 cell lysate Lane 7: K-562 cell lysate Lane 8: C6 cell lysate Lane 9: PC-12 cell lysate Lane 10: NIH/3T3 cell lysate Lane 11: RAW264.7 cell lysate Lane 12: Mouse testis tissue lysate Lane 13: Mouse kidney tissue lysate Lane 14: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 109 kDa Observed band size: 120 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32295) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32295_mouse-eftud2-mab-1-d10-a1-r-278202415212452.jpg "Western Blot: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Western blot analysis of EFTUD2 on different lysates with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HEK-293 cell lysate Lane 4: U-87 MG cell lysate Lane 5: LNCaP cell lysate Lane 6: HepG2 cell lysate Lane 7: K-562 cell lysate Lane 8: C6 cell lysate Lane 9: PC-12 cell lysate Lane 10: NIH/3T3 cell lysate Lane 11: RAW264.7 cell lysate Lane 12: Mouse testis tissue lysate Lane 13: Mouse kidney tissue lysate Lane 14: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 109 kDa Observed band size: 120 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32295) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Immunofluorescence analysis of paraffin-embedded human breast cancer tissue labeling EFTUD2 with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32295, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32295_mouse-eftud2-mab-1-d10-a1-r-298202414333649.jpg)
![Immunohistochemistry: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32295) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32295_mouse-eftud2-mab-1-d10-a1-r-29820241492628.jpg)
![Immunocytochemistry/ Immunofluorescence: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Immunocytochemistry analysis of HeLa cells labeling EFTUD2 with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32295_mouse-eftud2-mab-1-d10-a1-r-292024119238.jpg)
![Flow Cytometry: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Flow cytometric analysis of HeLa cells labeling EFTUD2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32295, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32295_mouse-eftud2-mab-1-d10-a1-r-2920241637218.jpg)
![Western Blot: EFTUD2 Antibody [NBP2-16282] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Western-Blot-NBP2-16282-img0002.jpg "Western Blot: EFTUD2 Antibody [NBP2-16282] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: EFTUD2 Antibody [NBP2-16282] - Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunohistochemistry-Paraffin-NBP2-16282-img0003.jpg)
![Western Blot: EFTUD2 Antibody [NBP2-16282] - Sample (30 ug of whole cell lysate) A: JurKat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Western-Blot-NBP2-16282-img0001.jpg)
