EDA2R/TNFRSF27/XEDAR Antibody (173103) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB, ELISA, ELISA(Cap), ELISA(Det)
• Clone: 173103
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

EDA2R/TNFRSF27/XEDAR Antibody (173103) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human EDA2R/TNFRSF27/XEDAR
  Met1-Glu136
  Accession # Q9HAV5
• Specificity: Detects human EDA2R/TNFRSF27/XEDAR in ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human (rh) 41BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR‑3, rhDR‑6, rhEDAR, rhFAS, rhGITR, rhHVEM, rhLTR‑b, rhNGFR, rhOPG, rhOX40 R, rhRANK, rhRELT, rhTAJ, rhTNF R1, rhTNF RII, rhTRADD, rhTRAIL R1, rhTRAIL R2, rhTRAIL R3, rhTRAIL R4, rhTWEAK R, recombinant mouse (rm) DcTRAIL R1, and rmDcTrail R2 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EDA2R
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • ELISA Standard (Matched Pair)
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for EDA2R/TNFRSF27/XEDAR Antibody (173103) [CoraFluor™ 1]

• ectodysplasin A2 receptor
• EDA2R
• EDA-A2 receptor
• EDAA2R
• EDA-A2R
• EDAA2Rtumor necrosis factor receptor superfamily member XEDAR
• EDAR2
• TNFRSF27
• XEDAR
• XEDARTNFRSF27tumor necrosis factor receptor superfamily member 27
• X-linked ectodysplasin-A2 receptor

Background

EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform. This protein is a type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily, and contains 3 cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. Multiple alternatively spliced transcript variants have been found for this gene, but some variants lack sufficient support.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EDA2R