ECT2 Antibody

Product Discontinued

ECT2 Antibody Summary

• Immunogen: Peptide with sequence AENSVLTSTTGRT-C corresponding to N-Terminus according to NP_060568.3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ECT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Approx. 100 kDa band reported in human U87-MG glioblastoma lysate at 2.5ug/mL. A 30 kDa band of unknown identity was also observed.
• Control:

  ECT2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ECT2 Antibody

• ARHGEF31
• epithelial cell transforming sequence 2 oncogene
• Epithelial cell-transforming sequence 2 oncogene
• FLJ10461
• MGC138291
• protein ECT2

Background

The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ECT2 Antibody (NB100-1259)(1)

Publication using NB100-1259

• Tatsumoto T, Xie X, Blumenthal R et al. Human ECT2 is an exchange factor for Rho GTPases, phosphorylated in G2/M phases, and involved in cytokinesis. J Cell Biol 1999-11-29 [PMID: 10579713]

Bioinformatics

• Gene Symbol: ECT2
• Entrez:
  • 1894(Human)
• Uniprot:
  • NP_060568.(Human)