ECM-1/Secretory Component P85 Antibody (SPM217) [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Rt
• Applications: Flow, ICC/IF, IHC, CyTOF-ready
• Clone: SPM217
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

ECM-1/Secretory Component P85 Antibody (SPM217) [Alexa Fluor™ Plus 647] Summary

• Immunogen: Secretory Component protein isolated from human colostrum (Uniprot: Q16610 )
• Localization: Cell surface and cytoplasmic
• Specificity: This monoclonal antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ECM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ECM-1/Secretory Component P85 Antibody (SPM217) [Alexa Fluor™ Plus 647]

• ECM1
• extracellular matrix protein 1
• p85
• Secretory Component Glycoprotein
• Secretory Component P85

Background

Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ECM1