ECM-1/Secretory Component P85 Antibody (SPM217) - IHC-Prediluted

Images

 
Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SPM217) - IHC-Prediluted [NBP2-44396] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with igA secretory component Monoclonal ...read more
Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SPM217) - IHC-Prediluted [NBP2-44396] - Formalin-fixed, paraffin-embedded normal human small intestine stained with Secretory Component MAb ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications IHC, IHC-P
Clone
SPM217
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

Order Details

ECM-1/Secretory Component P85 Antibody (SPM217) - IHC-Prediluted Summary

Immunogen
Secretory Component protein isolated from human colostrum
Localization
Cell surface and cytoplasmic
Specificity
This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ECM1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 1q21.2
Molecular weight of antigen: ~80kDa
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Alternate Names for ECM-1/Secretory Component P85 Antibody (SPM217) - IHC-Prediluted

  • ECM1
  • extracellular matrix protein 1
  • p85
  • Secretory Component Glycoprotein
  • Secretory Component P85

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34747AF350
Alexa Fluor 405 NBP2-34747AF405
Alexa Fluor 488 NBP2-34747AF488
Alexa Fluor 532 NBP2-34747AF532
Alexa Fluor 594 NBP2-34747AF594
Alexa Fluor 647 NBP2-34747AF647
Alexa Fluor 700 NBP2-34747AF700
Alexa Fluor 750 NBP2-34747AF750
Allophycocyanin NBP2-34747APC
Biotin NBP2-34747B
DyLight 350 NBP2-34747UV
DyLight 405 NBP2-34747V
DyLight 488 NBP2-34747G
DyLight 550 NBP2-34747R
DyLight 594 NBP2-34747DL594
DyLight 650 NBP2-34747C
DyLight 680 NBP2-34747FR
DyLight 755 NBP2-34747IR
FITC NBP2-34747F
HRP NBP2-34747H
Janelia Fluor 549 NBP2-34747JF549
Janelia Fluor 646 NBP2-34747JF646
PE NBP2-34747PE
PerCP NBP2-34747PCP

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Bioinformatics

Gene Symbol ECM1