ECM-1/Secretory Component P85 Antibody (SC05) - IHC-Prediluted

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) - IHC-Prediluted [NBP2-44395] - Formalin-fixed, paraffin-embedded normal human intestine (10X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) - IHC-Prediluted [NBP2-44395] - Formalin-fixed, paraffin-embedded normal human intestine (20X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) - IHC-Prediluted [NBP2-44395] - Staining of normal human small intestine. Note cytoplasmic and membrane staining.

Product Discontinued

ECM-1/Secretory Component P85 Antibody (SC05) - IHC-Prediluted Summary

• Immunogen: Partially purified ECM1 from human colostrum was used as immunogen to generate the antibody (Bartek, 1989).
• Localization: Cell surface and cytoplasmic
• Specificity: This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ECM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: 1q21.2
  Molecular weight of antigen: ~80kDa
  Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Alternate Names for ECM-1/Secretory Component P85 Antibody (SC05) - IHC-Prediluted

• ECM1
• extracellular matrix protein 1
• p85
• Secretory Component Glycoprotein
• Secretory Component P85

Background

Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ECM1