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ECM-1/Secretory Component P85 Antibody (rECM1/792)

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Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (rECM1/792) [NBP2-44397] - Human Colon Carcinoma stained with IgA Secretory Component Monoclonal Antibody (ECM1/792).
Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (rECM1/792) [NBP2-44397] - Human Colon Carcinoma stained with IgA Secretory Component Monoclonal Antibody (ECM1/792).
SDS-Page: ECM-1/Secretory Component P85 Antibody (rECM1/792) [NBP2-44397]

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications Flow, IHC, IHC-P, PAGE, ICC/IF
Clone
rECM1/792
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47804AF350
Alexa Fluor 405
NBP2-47804AF405
Alexa Fluor 488
NBP2-47804AF488
Alexa Fluor 532
NBP2-47804AF532
Alexa Fluor 594
NBP2-47804AF594
Alexa Fluor 647
NBP2-47804AF647
Alexa Fluor 700
NBP2-47804AF700
Alexa Fluor 750
NBP2-47804AF750
Allophycocyanin
NBP2-47804APC
Biotin
NBP2-47804B
DyLight 350
NBP2-47804UV
DyLight 405
NBP2-47804V
DyLight 488
NBP2-47804G
DyLight 550
NBP2-47804R
DyLight 594
NBP2-47804DL594
DyLight 650
NBP2-47804C
DyLight 680
NBP2-47804FR
DyLight 755
NBP2-47804IR
FITC
NBP2-47804F
HRP
NBP2-47804H
Janelia Fluor 549
NBP2-47804JF549
Janelia Fluor 646
NBP2-47804JF646
PE
NBP2-47804PE
PerCP
NBP2-47804PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-47804-0.1mg
IHC-Prediluted
NBP2-48176
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ECM-1/Secretory Component P85 Antibody (rECM1/792) Summary

Immunogen
Recombinant human ECM1 protein
Localization
Cell surface and cytoplasmic
Specificity
This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ECM1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • SDS-Page
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Hu-chromosome location: 1q21.2
Molecular weight of antigen: ~80kDa
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for ECM-1/Secretory Component P85 Antibody (rECM1/792)

  • ECM1
  • extracellular matrix protein 1
  • p85
  • Secretory Component Glycoprotein
  • Secretory Component P85

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in NIH 3T3 cell lysate.
Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Western Blot: Fibulin-3/EFEMP1 Antibody [NBP1-77040] - HeLa cell lysate with EFEMP1 antibody at (A) 0.5 and (B) 1 ug/ml.
Immunocytochemistry/Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP1-77040] - Immunofluorescence of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 ug/mL.
Immunocytochemistry/Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP1-77040] - HeLa cells with EFEMP1 antibody at 20 ug/ml.
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NBP1-88650
Western Blot: ARHGEF7 Antibody [NBP1-88650] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: ARHGEF7 Antibody [NBP1-88650] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
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PI 3-Kinase p85
alpha Antibody (6G10)
NBP2-22212
Western Blot: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis using PI 3 Kinase p85 alpha mAb against human PI 3 Kinase p85 alpha recombinant protein. (Expected MW is 53.4 kDa)
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of HepG2 cells using PI 3 Kinase p85 alpha mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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NBP2-34136
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NBP2-67504
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47804AF350
Alexa Fluor 405 NBP2-47804AF405
Alexa Fluor 488 NBP2-47804AF488
Alexa Fluor 532 NBP2-47804AF532
Alexa Fluor 594 NBP2-47804AF594
Alexa Fluor 647 NBP2-47804AF647
Alexa Fluor 700 NBP2-47804AF700
Alexa Fluor 750 NBP2-47804AF750
Allophycocyanin NBP2-47804APC
Biotin NBP2-47804B
DyLight 350 NBP2-47804UV
DyLight 405 NBP2-47804V
DyLight 488 NBP2-47804G
DyLight 550 NBP2-47804R
DyLight 594 NBP2-47804DL594
DyLight 650 NBP2-47804C
DyLight 680 NBP2-47804FR
DyLight 755 NBP2-47804IR
FITC NBP2-47804F
HRP NBP2-47804H
Janelia Fluor 549 NBP2-47804JF549
Janelia Fluor 646 NBP2-47804JF646
PE NBP2-47804PE
PerCP NBP2-47804PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol ECM1
Uniprot