ECH1 Antibody (5G8) - Azide and BSA Free

Western Blot: ECH1 Antibody (5G8) [H00001891-M01] - Analysis of ECH1 expression in transfected 293T cell line by ECH1 monoclonal antibody (M01), clone 5G8.Lane 1: ECH1 transfected lysate(35.8 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 5G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ECH1 Antibody (5G8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ECH1 (NP_001389, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GSNYPGLSISLRLTGSSAQEEASGVALGEAPDHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDL
• Specificity: ECH1 - enoyl Coenzyme A hydratase 1, peroxisomal
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ECH1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ECH1 Antibody (5G8) - Azide and BSA Free

• delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
• delta3,5-delta2,4-dienoyl-CoA isomerase
• dienoyl-CoA isomerase
• EC 5.3.3.-
• enoyl CoA hydratase 1, peroxisomal
• enoyl Coenzyme A hydratase 1, peroxisomal
• HPXEL
• peroxisomal enoyl-CoA hydratase 1

Background

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ECH1 Antibody (H00001891-M01)(1)

Publication using H00001891-M01

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: ECH1
• Entrez:
  • 1891(Human)
• OMIM:
  • 600696(Human)
• Uniprot:
  • NP_001389(Human)