EB3 Antibody (JU38-30)

Western Blot: EB3 Antibody (JU38-30) [NBP2-75477] - Analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution.Positive control:Lane 1: Mouse brain tissue Lane 2: K562

Immunocytochemistry/Immunofluorescence: EB3 Antibody (JU38-30) [NBP2-75477] - Staining EB3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: EB3 Antibody (JU38-30) [NBP2-75477] - Analysis of paraffin-embedded rat cerebellum tissue using anti-EB3 antibody. Counter stained with hematoxylin.

Flow Cytometry: EB3 Antibody (JU38-30) [NBP2-75477] - Analysis of SH-SY-5Y cells with EB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: EB3 Antibody (JU38-30) [NBP2-75477] - Staining EB3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: EB3 Antibody (JU38-30) [NBP2-75477] - Staining EB3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: EB3 Antibody (JU38-30) [NBP2-75477] - Analysis of paraffin-embedded human lung cancer tissue using anti-EB3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: EB3 Antibody (JU38-30) [NBP2-75477] - Analysis of paraffin-embedded mouse brain tissue using anti-EB3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: EB3 Antibody (JU38-30) [NBP2-75477] - Analysis of paraffin-embedded mouse fallopian tubes tissue using anti-EB3 antibody. Counter stained with hematoxylin.

Product Discontinued

EB3 Antibody (JU38-30) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human EB3 aa 165-281 / 281. (SwissProt: Q9UPY8 Human; SwissProt: Q6PER3 Mouse; SwissProt: Q5XIT1 Rat)
• Localization: Cytoskeleton.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAPRE3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for EB3 Antibody (JU38-30)

• EB1 protein family member 3
• EB3EBF3-S
• End-binding protein 3
• microtubule-associated protein RP/EB family member 3
• microtubule-associated protein, RP/EB family, member 3
• RP3EBF3APC binding protein

Background

EB3 is a 282 amino acid homologue of EB1, a protein known to bind APC (adenomatous polyposis coli). It has been shown to bind to tubulin in vitro and microtubules in vivo. Northern blotting shows expressions to be mainly in brain, and to a lesser degree in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPRE3