E2F-2 Antibody

Images

 
Western Blot: E2F-2 Antibody [H00001870-D01P] - Analysis of E2F2 expression in transfected 293T cell line by E2F2 polyclonal antibody.Lane 1: E2F2 transfected lysate(47.50 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Assay: E2F-2 Antibody [H00001870-D01P] - Analysis of protein-protein interactions between E2F2 and E2F1. HeLa cells were stained with anti-E2F2 rabbit purified polyclonal 1:1200 and anti-E2F1 mouse ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

E2F-2 Antibody Summary

Immunogen
E2F2 (AAH53676.1, 1 a.a. - 437 a.a.) full-length human protein. MLQGPRALASAAGQTPKVVPAMSPTELWPSGLSSPQLCPATATYYTPLYPQTAPPAAAPGTCLDATPHGPEGQVVRCLPAGRLPAKRKLDLEGIGRPVVPEFPTPKGKCIRVDGLPSPKTPKSPGEKTRYDTSLGLLTKKFIYLLSESEDGVLDLNWAAEVLDVQKRRIYDITNVLEGIQLIRKKAKNNIQWVGRGMFEDPTRPGKQQQLGQELKELMNTEQALDHLIQSCSLSFKHLTEDKANKRLAYVTYQDIRAVGNFKEQTVIAVKAPPQTRLEVPDRTEDNLQIYLKSTQGPIEVYLCPEEVQEPDSPSEEPLPSTSTLCPSPDSAQPSSSTDPSIMEPTASSVPAPAPTPQQAPPPPSLVPLEATDSLLELPHPLLQQTEDQFLSPTLACSSPLISFSPSLDQDDYLWGLEAGEGISDLFDSYDLGDLLIN
Specificity
E2F transcription factor 2
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
E2F2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Proximity Ligation Assay
  • Western Blot
Application Notes
This antibody is reactive against mammalian transfected lysate in WB. It has been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for E2F-2 Antibody

  • E2F transcription factor 2
  • E2F2
  • E2F-2
  • E2F-2transcription factor E2F2

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00001870-D01P
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Applications: WB, PLA

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol E2F2
Entrez
Uniprot