HAF017). A specific band for E2F-2 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human E2F-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for E2F-2 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5119-SP | |||
![Western Blot: E2F-2 Antibody (1H7D6) [NBP3-16165] - Western blot analysis of extracts of various cell lines, using E2F-2 Rabbit mAb (NBP3-16165) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/E2F-2-Antibody-1H7D6-Western-Blot-NBP3-16165-img0001.jpg "Western Blot: E2F-2 Antibody (1H7D6) [NBP3-16165] - Western blot analysis of extracts of various cell lines, using E2F-2 Rabbit mAb (NBP3-16165) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Western Blot: E2F-2 Antibody [NBP3-10478] - Western blot analysis using NBP3-10478 on Human brain as a positive control. Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/E2F-2-Antibody-Western-Blot-NBP3-10478-img0001.jpg "Western Blot: E2F-2 Antibody [NBP3-10478] - Western blot analysis using NBP3-10478 on Human brain as a positive control. Antibody Titration: 0.2-1 ug/ml")
![Western Blot: E2F-2 Antibody [H00001870-D01P] - Analysis of E2F2 expression in transfected 293T cell line by E2F2 polyclonal antibody.Lane 1: E2F2 transfected lysate(47.50 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/E2F-2-Antibody-Western-Blot-H00001870-D01P-img0002.jpg "Western Blot: E2F-2 Antibody [H00001870-D01P] - Analysis of E2F2 expression in transfected 293T cell line by E2F2 polyclonal antibody.Lane 1: E2F2 transfected lysate(47.50 KDa).Lane 2: Non-transfected lysate.")
![Proximity Ligation Assay: E2F-2 Antibody [H00001870-D01P] - Analysis of protein-protein interactions between E2F2 and E2F1. HeLa cells were stained with anti-E2F2 rabbit purified polyclonal 1:1200 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/E2F-2-Antibody-Proximity-Ligation-Assay-H00001870-D01P-img0001.jpg)
![Western Blot: E2F-2 Antibody [H00001870-B01P] - Analysis of E2F2 expression in transfected 293T cell line by E2F2 polyclonal antibody. Lane 1: E2F2 transfected lysate(9.24 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/E2F-2-Antibody-Western-Blot-H00001870-B01P-img0002.jpg "Western Blot: E2F-2 Antibody [H00001870-B01P] - Analysis of E2F2 expression in transfected 293T cell line by E2F2 polyclonal antibody. Lane 1: E2F2 transfected lysate(9.24 KDa). Lane 2: Non-transfected lysate.")
