E2F-1 Antibody

Western Blot: E2F-1 Antibody [NBP1-31394] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: E2F-1 Antibody [NBP1-31394] - Analysis of HeLa, using NBP1-31394 at 1:200 dilution.

Western Blot: E2F-1 Antibody [NBP1-31394] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE, antibody diluted at 1:500.

Product Discontinued

E2F-1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human E2F1. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: E2F1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for E2F-1 Antibody

• E2F transcription factor 1
• E2F1
• E2F-1
• PBR3
• PRB-binding protein E2F-1
• RBAP1
• RBAP-1
• RBBP3
• RBBP-3
• RBP3
• Retinoblastoma-associated protein 1
• Retinoblastoma-binding protein 3
• transcription factor E2F1

Background

E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: E2F1
• Entrez:
  • 1869(Human)
  • 13555(Mouse)
• Uniprot:
  • Q01094()