E2F-1 Antibody [mFluor Violet 450 SE]

• Reactivity: Hu
• Applications: WB, IHC, ELISA(Cap)
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: mFluor Violet 450 SE

E2F-1 Antibody [mFluor Violet 450 SE] Summary

• Immunogen: E. coli-derived recombinant human E2F-1
  Val264-Phe437
  Accession # Q01094
• Specificity: Detects human E2F-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human E2F-2, -3, -4, -5, -6, -7, and -8 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: E2F1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for E2F-1 Antibody [mFluor Violet 450 SE]

• E2F transcription factor 1
• E2F1
• E2F-1
• PBR3
• PRB-binding protein E2F-1
• RBAP1
• RBAP-1
• RBBP3
• RBBP-3
• RBP3
• Retinoblastoma-associated protein 1
• Retinoblastoma-binding protein 3
• transcription factor E2F1

Background

E2F1 is encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: E2F1