Dystroglycan Antibody [DyLight 405]

• Reactivity: Hu
• Applications: WB, Flow, IHC, KO
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: DyLight 405

Dystroglycan Antibody [DyLight 405] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Dystroglycan
  Gln28-Val749
  Accession # Q14118
• Specificity: Detects human Dystroglycan in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DAG1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Knockout Validated
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product AF6868. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Dystroglycan Antibody [DyLight 405]

• A3a
• AGRNR
• DAG1
• Dag-1
• DAG156DAG
• dystroglycan 1 (dystrophin-associated glycoprotein 1)
• Dystroglycan
• Dystrophin-associated glycoprotein 1

Background

Dystroglycan (also known as dystrophin-associated glycoprotein 1) is a member of the dystroglycan family that contains the WWW binding motif PPxY. This protein has alpha and beta subunits with approximate molecular weights of 156 kD and 43-50 kD, respectively. The dystroglycan beta subunit is an integral membrane protein, the alpha subunit is a ubiquitously expressed extracellular protein. Muscle and non-muscle isoforms differ by carbohydrate moieties (not protein sequence). Dystroglycan functions as an adhesion molecule responsible for interactions between extracellular matrix and the subsarcolemmal cytoskeleton. Dsytroglycan binds to lamin in the matrix and dystrophin in the cytoskeleton; phosphorylation regulates the association of interacting proteins. Dystroglycan binds to utrophin when unphosphorylated; c-Src, Fyn, Csk, NCK, and SHC when phosphorylated. Dystroglycan interacts with dystrophin through the WW domain. The Poly6171 antibody recognizes human phosphorylated dystroglycan (Tyr893) and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DAG1