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Dystrobrevin alpha Antibody

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Western Blot: Dystrobrevin alpha Antibody [NBP2-92053] - Analysis of extracts of various cell lines, using Dystrobrevin alpha at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Western Blot: Dystrobrevin alpha Antibody [NBP2-92053] - Analysis of extracts of various cell lines, using Dystrobrevin alpha at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Dystrobrevin alpha Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 240-480 of human DTNA (NP_001381.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DTNA
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Dystrobrevin alpha Antibody

  • Alpha-dystrobrevin
  • D18S892EDTN
  • DRP3FLJ96209
  • DTN-1
  • DTN-2
  • DTN-3
  • DTN-A
  • dystrobrevin alpha
  • dystrobrevin, alpha
  • Dystrophin-related protein 3
  • LVNC1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: Dystrophin Antibody [NBP1-89953] - Staining of human heart muscle shows strong membranous positivity in myocytes.
Immunohistochemistry-Paraffin: Dystrophin Antibody [NBP1-89953] - Staining of human skeletal muscle shows strong membranous positivity in myocytes.
Immunohistochemistry-Paraffin: Dystrophin Antibody [NBP1-89953] - Staining of human tonsil shows no positivity as expected.
(4 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
Utrophin Antibody
NBP1-84074
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human prostate shows moderate to strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli and cells in tubules.
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human stomach shows moderate membranous positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
LDB3 Antibody (3C8)
H00011155-M06
Western Blot: LDB3 Antibody (3C8) [H00011155-M06] - Western blot analysis of LDB3 over-expressed 293 cell line, cotransfected with LDB3 Validated Chimera RNAi or non-transfected control. Blot probed with H00011155-M06. GAPDH (36.1 kDa) used as loading control.
ELISA: LDB3 Antibody (3C8) [H00011155-M06] - Detection limit for recombinant GST tagged LDB3 is approximately 0.03ng/ml as a capture antibody.
Immunocytochemistry/Immunofluorescence: LDB3 Antibody (3C8) [H00011155-M06] - Analysis of monoclonal antibody to LDB3 on A-431 cell. Antibody concentration 10 ug/ml
(3 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IP, KD, WB
Dystrobrevin beta
Antibody (OTI3A12)
NBP2-01798
Flow Cytometry: Dystrobrevin beta Antibody (3A12) [NBP2-01798] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Dystrobrevin beta antibody, and then analyzed by flow cytometry.
Western Blot: Dystrobrevin beta Antibody (3A12) [NBP2-01798] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dystrobrevin beta (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Dystrobrevin beta.
Immunohistochemistry-Paraffin: Dystrobrevin beta Antibody (3A12) [NBP2-01798] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Dystrobrevin beta mouse monoclonal antibody.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
XCL1/Lymphotactin
486-LT/CF
XCL1/Lymphotactin
(8 Publications)
Species: Mu
Applications: BA
TAZ/WWTR1 Antibody
- BSA Free
NB110-58359
Simple Western: TAZ/WWTR1 Antibody [NB110-58359] - Simple Western lane view shows a specific band for TAZ in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunocytochemistry/Immunofluorescence: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ (Green) in HepG2 cells using NB110-58359. Nuclei (Blue) are counterstained with Hoecsht 33258.
Western Blot: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ on HEK 293 cell lysate using NB110-58359.
(1 Review)
(13 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, PLA, Simple Western, WB

Acetylcholinesterase/A
NB100-1519
Flow Cytometry: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Staining (0.3ug/ml) of Jurkat (A) and (0.5ug/ml) HepG2 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-FrFl, PEP-ELISA, WB
GLYAT Antibody
NBP2-58917
Western Blot: GLYAT Antibody [NBP2-58917] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human duodenum shows low expression as expected.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human kidney shows high expression.
Species: Hu
Applications: IHC, IHC-P, WB
CRAT Antibody
NBP1-86615
Immunohistochemistry-Paraffin: CRAT Antibody [NBP1-86615] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: CRAT Antibody [NBP1-86615] - Staining of human tonsil shows low expression as expected.
Western Blot: CRAT Antibody [NBP1-86615] - Staining in human testis and tonsil tissues using anti-CRAT antibody. Corresponding CRAT RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P, WB
Catalase Antibody
[Unconjugated]
AF3398
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, A20 mouse B cell lymphoma cell line, andRat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Catalase at approximately 64 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. 
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Catalase at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Catalase at approximately 64 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(7 Reviews)
(5 Publications)
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB

Butyrylcholinesterase/
NBP2-89134
Western Blot: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Analysis of BCHE on human blood plasma using anti-BCHE antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Staining BCHE in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Staining BCHE in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
TTC11 Antibody
NB100-56646
Western Blot: TTC11 Antibody [NB100-56646] - Effect of liraglutide on brain cortical mitochondrial fission/fusion markers in female mice with early AD-like pathology. Brain cortical Fis1 (A) protein levels were determined and normalized to beta-actin levels, and representative Western blotting images displayed. Data are the mean +/- SE from 6 mice/group. Statistical significance: * p < 0.05, by the one-way ANOVA with the Fisher LSD post-hoc test for multiple comparisons. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/21/5/1746), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: TTC11 Antibody [NB100-56646] - Analysis of methcarn-fixed, paraffin-embedded hippocampus from control (normal) and Alzheimer brain. Quantification in five AD brains and five age-matched controls indicated that Fis1 was detected in only the soma in ~80% of pyramidal neurons in AD hippocampus, significantly different from what was seen for control hippocampal neurons. Source: Wang et al, 2009.
Knockdown Validated: TTC11 Antibody [NB100-56646] - Analysis of Fis1 in A) human HeLa and B) mouse NIH 3T3 lysate using Fis1 antibody at 1:1000 dilution. B. Specificity validation of the Fis1 antibody. Fis1 was analyzed in M17 cells stably transfected with vector only, Fis1 RNAi, Fis1 (Fis1wt OE) or left untransfected (negative control). The western blot data showed that M17 cells expressed Fis1 (Vector, Untransfected control) which could be knocked down by Fis1 RNAi. Cells transfected with Fis1 overexpressed Fis1. M17 is a human neuroblastoma cell line.
(26 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
Lamin A + C
Antibody (4C4)
NBP2-25151
Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of HeLa cells stained with mouse mAb to lamin A/C, NBP2-25151, dilution 1:2,000 in red, and costained with rabbit pAb to HSP60, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. NBP2-25151 antibody specifically labels the nuclear lamina, while the HSP60 antibody reveals protein expressed in mitochondria.
Western Blot: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of different cell lysates using mouse mAb to lamin A/C, NBP2-25151, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. NBP2-25151 antibody failed to recognize rat or mouse proteins.
Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - HeLa cells staing with NBP2-25151 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25151 reveals strong nuclear lamina staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments.
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, WB
Tight Junction
Protein 1 Antibody
NBP1-85047
Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of Pig trabecular meshwork cells. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.
(5 Reviews)
(10 Publications)
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Dystroglycan
Antibody
NBP2-14868
Western Blot: Dystroglycan Antibody [NBP2-14868] - U87-MG whole cell extracts and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAG1 antibody diluted at 1:500.
Immunocytochemistry/Immunofluorescence: Dystroglycan Antibody [NBP2-14868] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dystroglycan protein stained by Dystroglycan antibody 38) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Dystroglycan Antibody [NBP2-14868] - Mouse placenta. DAG1 stained by Dystroglycan antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
(2 Publications)
Species: Eq, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MyBPC3 Antibody
AF7439
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MyBPC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7439) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MyBPC3 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
MyBPC3 was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human MyBPC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7439) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: IHC, WB
FKBP12 Antibody
(422513)
MAB3777
Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat FKBP12 Monoclonal Antibody (Catalog # MAB3777) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human FKBP12 (Catalog # 3777-FK), recombinant human FKBP12.6, and recombinant human FKBP13 (5 ng/lane) were included. A specific band for FKBP12 was detected at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB
Dystrobrevin alpha
Antibody
NBP2-92053
Dystrobrevin alpha Antibody
Species: Hu, Mu
Applications: WB

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Bioinformatics

Gene Symbol DTNA