Dyrk1B Overexpression Lysate (Native)

Images

 
Western Blot: Dyrk1B Overexpression Lysate (Adult Normal) [NBL1-10075] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dyrk1B.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Dyrk1B Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205861 and based on accession number NM_004714. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B), transcript variant a, mRNA.
Gene
DYRK1B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Dyrk1B

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Dyrk1B Overexpression Lysate (Native)

  • dual specificity tyrosine-phosphorylation-regulated kinase 1B
  • dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B
  • EC 2.7.12
  • Minibrain-related kinase
  • Mirk protein kinase
  • MIRKEC 2.7.12.1

Background

DYRK1B is a member of the DYRK family of protein kinases. DYRK1B contains a bipartite nuclear localization signal and is found mainly in muscle and testis. The protein is proposed to be involved in the regulation of nuclear functions. Three isoforms of DYRK1B have been identified differing in the presence of two alternatively spliced exons within the catalytic domain. [provided by RefSeq]. Transcript Variant: This splice variant contains all exons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00001859-M01
Species: Hu
Applications: WB, ELISA, IHC, IP
NBP2-52508
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC, IF
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
NBP2-15141
Species: Hu
Applications: WB
NBP2-47560
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-80616
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-84745
Species: Hu
Applications: IHC, IHC-P
NBP2-13387
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF1555
Species: Hu
Applications: WB
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-92589
Species: Hu, Dr
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82455
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56510
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-15087
Species: Hu, Mu
Applications: WB
NBP2-31376
Species: Hu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
NB110-94452
Species: Hu, Ch, Mu(-), Rt(-)
Applications: WB, ICC/IF
NBP2-16521
Species: Hu, Mu
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NB100-56092
Species: Hu
Applications: WB, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBL1-10075
Species: Hu
Applications: WB

Publications for Dyrk1B Lysate (NBL1-10075) (0)

There are no publications for Dyrk1B Lysate (NBL1-10075).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Dyrk1B Lysate (NBL1-10075) (0)

There are no reviews for Dyrk1B Lysate (NBL1-10075). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Dyrk1B Lysate (NBL1-10075) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Dyrk1B Products

Bioinformatics Tool for Dyrk1B Lysate (NBL1-10075)

Discover related pathways, diseases and genes to Dyrk1B Lysate (NBL1-10075). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Dyrk1B Lysate (NBL1-10075)

Discover more about diseases related to Dyrk1B Lysate (NBL1-10075).
 

Pathways for Dyrk1B Lysate (NBL1-10075)

View related products by pathway.

PTMs for Dyrk1B Lysate (NBL1-10075)

Learn more about PTMs related to Dyrk1B Lysate (NBL1-10075).
 

Research Areas for Dyrk1B Lysate (NBL1-10075)

Find related products by research area.

Blogs on Dyrk1B

There are no specific blogs for Dyrk1B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Dyrk1B Antibody
NBP1-33464

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Dyrk1B Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DYRK1B