Dynein intermediate chain 2 Antibody (1C8) - Azide and BSA Free

Western Blot: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - DNAI2 monoclonal antibody (M01), clone 1C8 Analysis of DNAI2 expression in A-431.

Immunocytochemistry/Immunofluorescence: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - Analysis of monoclonal antibody to DNAI2 on A-431 cell. Antibody concentration 10 ug/ml.

Western Blot: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - Analysis of DNAI2 expression in transfected 293T cell line by DNAI2 monoclonal antibody (M01), clone 1C8.Lane 1: DNAI2 transfected lysate(67 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - Detection limit for recombinant GST tagged DNAI2 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Ca, Re
• Applications: WB, ELISA, Func, ICC/IF
• Clone: 1C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Dynein intermediate chain 2 Antibody (1C8) - Azide and BSA Free Summary

• Immunogen: DNAI2 (AAH39582, 1 a.a. ~ 593 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEIVYVYVKKRSEFGKQCNFSDRQAELNIDIMPNPELAEQFVERNPVDTGIQCSISMSEHEANSERFEMETRGVNHVEGGWPKDVNPLELEQTIRFRKKVEKDENYVNAIMQLGSIMEHCIKQNNAIDIYEEYFNDEEAMEVMEEDPSAKTINVFRDPQEIKRAATHLSWHPDGNRKLAVAYSCLDFQRAPVGMSSDSYIWDLENPNKPELALKPSSPLVTLEFNPKDSHVLLGGCYNGQIACWDTRKGSLVAELSTIESSHRDPVYGTIWLQSKTGTECFSASTDGQVMWWDIRKMSEPTEVVILDITKKEQLENALGAISLEFESTLPTKFMVGTEQGIVISCNRKAKTSAEKIVCTFPGHHGPIYALQRNPFYPKNFLTVGDWTARIWSEDSRESSIMWTKYHMAYLTDAAWSPVRPTVFFTTRMDGTLDIWDFMFEQCDPTLSLKDNGCLIACGSQLGTTTLLEVSPGLSTLQRNEKNVASSMFERETRREKILEARHREMRLKEKGKAEGRDEEQTDEELAVDLEALVSKAEEEFFDIIFTELKKKEADAIKLTPVPQQPSPEEDQVVEEGEEAAGEEGDEEVEEDLA
• Specificity: DNAI2 - dynein, axonemal, intermediate polypeptide 2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DNAI2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Functional
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Dynein intermediate chain 2 Antibody (1C8) - Azide and BSA Free

• Axonemal dynein intermediate chain 2
• CILD9
• dynein intermediate chain 2, axonemal
• dynein, axonemal, intermediate chain 2
• dynein, axonemal, intermediate polypeptide 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dynein intermediate chain 2 Antibody (H00064446-M01)(16)

Publications using H00064446-M01

• Kim DY, Sub YJ, Kim HY et al. LRRC6 regulates biogenesis of motile cilia by aiding FOXJ1 translocation into the nucleus Cell communication and signaling : CCS 2023-06-16 [PMID: 37328841] (Western Blot, Immunocytochemistry/ Immunofluorescence, Mouse)
• Takahiro I, Wang T, Hao L et al. CFAP53 regulates mammalian cilia-type motility patterns through differential localization and recruitment of axonemal dynein components. PLoS Genet. 2020-12-21 [PMID: 33347437]
• Kajikawa E, Horo U, Ide T et al. Nodal paralogues underlie distinct mechanisms for visceral left-right asymmetry in reptiles and mammals. Nat Ecol Evol. 2020-01-06 [PMID: 31907383]
• Beurois J, Martinez G, Cazin C et al. CFAP70 mutations lead to male infertility due to severe astheno-teratozoospermia. A case report. Hum Reprod. 2019-10-02 [PMID: 31621862]
• Li Y, Sha Y, Wang X et al. DNAH2 is a novel candidate gene associated with multiple morphological abnormalities of the sperm flagella Clin. Genet. 2019-02-27 [PMID: 30811583] (ICC/IF, WB, Human)
• Zhang Y, Chen Y, Zheng J et al. Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat. J Mol Cell Biol 2018-07-28 [PMID: 30060180]
• Cho KJ, Noh SH, Han SM et al. ZMYND10 stabilizes intermediate chain proteins in the cytoplasmic pre-assembly of dynein arms. PLoS Genet. 2018-03-30 [PMID: 29601588]
• Miao C, Jiang Q, Li H et al. Mutations in the Motile Cilia Gene DNAAF1 Are Associated with Neural Tube Defects in Humans. G3 (Bethesda) 2016-10-13 [PMID: 27543293] (Human)
• Kourtidis A, Anastasiadis PZ. PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications. Cell Cycle. 2016-01-29 [PMID: 26822694] (ICC/IF, Human)
• Diggle CP, Moore DJ, Mali G et al. HEATR2 Plays a Conserved Role in Assembly of the Ciliary Motile Apparatus. PLoS Genet. 2014-09-18 [PMID: 25232951]
• Dong F, Shinohara K, Botilde Y et al. Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm. J Cell Biol. 2014-01-13 [PMID: 24421334]
• Ben Khelifa M, Coutton C, Zouari R et al. Mutations in DNAH1, which Encodes an Inner Arm Heavy Chain Dynein, Lead to Male Infertility from Multiple Morphological Abnormalities of the Sperm Flagella. Am J Hum Genet. 2013-12-19 [PMID: 24360805]
• Tarkar A, Loges NT, Slagle CE et al. DYX1C1 is required for axonemal dynein assembly and ciliary motility. Nat Genet 2013-09-01 [PMID: 23872636]
• Astanina K, Jacob Ret al. KIF5C, a kinesin motor involved in apical trafficking of MDCK cells. Cell Mol Life Sci 2010-04-01 [PMID: 20094756] (WB, Canine)
• Omran H, Kobayashi D, Olbrich H et al. Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins. Nature. 2008-12-04 [PMID: 19052621]
• Loges NT, Olbrich H, Fenske L et al. DNAI2 Mutations Cause Primary Ciliary Dyskinesia with Defects in the Outer Dynein Arm. Am J Hum Genet. 2008-10-22 [PMID: 18950741]

Bioinformatics

• Gene Symbol: DNAI2
• Entrez:
  • 64446(Human)
• OMIM:
  • 605483(Human)
• Uniprot:
  • AAH39582(Human)