Dynein intermediate chain 2 Antibody (1C8)

Images

 
Western Blot: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - DNAI2 monoclonal antibody (M01), clone 1C8 Analysis of DNAI2 expression in A-431.
Immunocytochemistry/ Immunofluorescence: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - Analysis of monoclonal antibody to DNAI2 on A-431 cell. Antibody concentration 10 ug/ml.
Western Blot: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - Analysis of DNAI2 expression in transfected 293T cell line by DNAI2 monoclonal antibody (M01), clone 1C8.Lane 1: DNAI2 transfected lysate(67 ...read more
Sandwich ELISA: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - Detection limit for recombinant GST tagged DNAI2 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, Ca, ReSpecies Glossary
Applications WB, ELISA, Func, ICC/IF, S-ELISA
Clone
1C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Dynein intermediate chain 2 Antibody (1C8) Summary

Immunogen
DNAI2 (AAH39582, 1 a.a. ~ 593 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEIVYVYVKKRSEFGKQCNFSDRQAELNIDIMPNPELAEQFVERNPVDTGIQCSISMSEHEANSERFEMETRGVNHVEGGWPKDVNPLELEQTIRFRKKVEKDENYVNAIMQLGSIMEHCIKQNNAIDIYEEYFNDEEAMEVMEEDPSAKTINVFRDPQEIKRAATHLSWHPDGNRKLAVAYSCLDFQRAPVGMSSDSYIWDLENPNKPELALKPSSPLVTLEFNPKDSHVLLGGCYNGQIACWDTRKGSLVAELSTIESSHRDPVYGTIWLQSKTGTECFSASTDGQVMWWDIRKMSEPTEVVILDITKKEQLENALGAISLEFESTLPTKFMVGTEQGIVISCNRKAKTSAEKIVCTFPGHHGPIYALQRNPFYPKNFLTVGDWTARIWSEDSRESSIMWTKYHMAYLTDAAWSPVRPTVFFTTRMDGTLDIWDFMFEQCDPTLSLKDNGCLIACGSQLGTTTLLEVSPGLSTLQRNEKNVASSMFERETRREKILEARHREMRLKEKGKAEGRDEEQTDEELAVDLEALVSKAEEEFFDIIFTELKKKEADAIKLTPVPQQPSPEEDQVVEEGEEAAGEEGDEEVEEDLA
Specificity
DNAI2 - dynein, axonemal, intermediate polypeptide 2
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DNAI2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Publications
Read Publications using
H00064446-M01 in the following applications:

  • 2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Dynein intermediate chain 2 Antibody (1C8)

  • Axonemal dynein intermediate chain 2
  • CILD9
  • dynein intermediate chain 2, axonemal
  • dynein, axonemal, intermediate chain 2
  • dynein, axonemal, intermediate polypeptide 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dynein intermediate chain 2 Antibody (H00064446-M01)(12)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(2)
WB
(2)
All Applications
Filter By Species
Human
(3)
Canine
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using H00064446-M01 Applications Species
Li Y, Sha Y, Wang X et al. DNAH2 is a novel candidate gene associated with multiple morphological abnormalities of the sperm flagella Clin. Genet. Feb 27 2019 [PMID: 30811583] (ICC/IF, WB, Human) ICC/IF, WB Human
Zhang Y, Chen Y, Zheng J et al. Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat. J Mol Cell Biol Jul 28 2018 [PMID: 30060180]
Cho KJ, Noh SH, Han SM et al. ZMYND10 stabilizes intermediate chain proteins in the cytoplasmic pre-assembly of dynein arms. PLoS Genet. 2018 Mar 30 [PMID: 29601588]
Miao C, Jiang Q, Li H et al. Mutations in the Motile Cilia Gene DNAAF1 Are Associated with Neural Tube Defects in Humans. G3 (Bethesda) 2016 Oct 13 [PMID: 27543293] (Human) Human
Kourtidis A, Anastasiadis PZ. PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications. Cell Cycle. 2016 Jan 29 [PMID: 26822694] (ICC/IF, Human) ICC/IF Human
Diggle CP, Moore DJ, Mali G et al. HEATR2 Plays a Conserved Role in Assembly of the Ciliary Motile Apparatus. PLoS Genet. 2014 Sep 18 [PMID: 25232951]
Dong F, Shinohara K, Botilde Y et al. Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm. J Cell Biol. 2014 Jan 13 [PMID: 24421334]
Ben Khelifa M, Coutton C, Zouari R et al. Mutations in DNAH1, which Encodes an Inner Arm Heavy Chain Dynein, Lead to Male Infertility from Multiple Morphological Abnormalities of the Sperm Flagella. Am J Hum Genet. 2013 Dec 19 [PMID: 24360805]
Tarkar A, Loges NT, Slagle CE et al. DYX1C1 is required for axonemal dynein assembly and ciliary motility. Nat Genet. 2013 Sep [PMID: 23872636]
Astanina K, Jacob Ret al. KIF5C, a kinesin motor involved in apical trafficking of MDCK cells. Cell Mol Life Sci 2010 Apr [PMID: 20094756] (WB, Canine) WB Canine
Show All 12 Publications.

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Bioinformatics

Gene Symbol DNAI2
Entrez
OMIM
Uniprot