Dynamin 3 Antibody

Immunohistochemistry-Paraffin: Dynamin 3 Antibody [NB100-93449] - (2.5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Dynamin 3 Antibody Summary

• Immunogen: Peptide with sequence C-DKSVGNNKAEND corresponding to internal region according to NP_056384.2.
• Epitope: C-DKSVGNNKAEND
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DNM3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:1000
• Application Notes: WB: Preliminary experiments gave an approx. 26 kDa band in human brain (amygdala and cerebellum), kidney and liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.1 kDa band according to NP_056384.2. The 26 kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  Dynamin 3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Dynamin 3 Antibody

• Dyna III
• dynamin 3
• dynamin family member
• dynamin-3
• EC 3.6.5.5
• MGC70433
• T-dynamin
• testicular

Background

Members of the dynamin family, such as DNM3, possess mechanochemical properties involved in actin-membrane processes, predominantly in membrane budding (Orth and McNiven, 2003 [PubMed 12517701]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dynamin 3 Antibody (NB100-93449)(1)

Publication using NB100-93449

• Ferguson SM, Brasnjo G, Hayashi M et al. A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Science 2007-04-27 [PMID: 17463283]

Bioinformatics

• Gene Symbol: DNM3
• Entrez:
  • 26052(Human)
• Uniprot:
  • NP_056384.2(Human)