Dynamin 3 Overexpression Lysate (Native)

Images

 
Western Blot: Dynamin 3 Overexpression Lysate (Adult Normal) [NBL1-09969] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dynamin 3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Dynamin 3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC224326 and based on accession number NM_015569. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens dynamin 3 (DNM3), transcript variant 1, mRNA.
Gene
DNM3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
96.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Dynamin 3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Dynamin 3 Overexpression Lysate (Native)

  • Dyna III
  • dynamin 3
  • dynamin family member
  • dynamin-3
  • EC 3.6.5.5
  • MGC70433
  • T-dynamin
  • testicular

Background

Members of the dynamin family, such as DNM3, possess mechanochemical properties involved in actin-membrane processes, predominantly in membrane budding (Orth and McNiven, 2003 [PubMed 12517701]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-47477
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB110-60491
Species: Hu, Mu
Applications: WB, Simple Western, ELISA, Flow, IHC, IHC-Fr, IHC-P
NB110-55288
Species: Hu, Mu, Rt, Fi, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
AF-258-PB
Species: Hu
Applications: WB, Simple Western, IHC
NBP2-59376
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IP
AF1556
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
AF8516
Species: Hu
Applications: WB, Simple Western, IP, ICC
NBP1-44999
Species: Hu, Mu
Applications: WB, ICC/IF
NBP2-37364
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, ICC
NBP1-82580
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P
H00010097-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NB600-600
Species: Hu, Mu
Applications: WB, ChIP, ELISA, IHC, IHC-P, Flow-IC
AF3184
Species: Hu, Mu
Applications: WB, Simple Western, ICC
NB110-55290
Species: Hu, Mu, Rt, Po, Ch
Applications: WB, Simple Western, IHC, IHC-P

Publications for Dynamin 3 Lysate (NBL1-09969) (0)

There are no publications for Dynamin 3 Lysate (NBL1-09969).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Dynamin 3 Lysate (NBL1-09969) (0)

There are no reviews for Dynamin 3 Lysate (NBL1-09969). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Dynamin 3 Lysate (NBL1-09969) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Dynamin 3 Products

Bioinformatics Tool for Dynamin 3 Lysate (NBL1-09969)

Discover related pathways, diseases and genes to Dynamin 3 Lysate (NBL1-09969). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Dynamin 3 Lysate (NBL1-09969)

Discover more about diseases related to Dynamin 3 Lysate (NBL1-09969).
 

Pathways for Dynamin 3 Lysate (NBL1-09969)

View related products by pathway.

Research Areas for Dynamin 3 Lysate (NBL1-09969)

Find related products by research area.

Blogs on Dynamin 3

There are no specific blogs for Dynamin 3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

DRP1 Antibody
NB110-55288
OPA1 Antibody
NB110-55290

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Dynamin 3 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DNM3