dUTPase Antibody (1C9)

Product Discontinued

dUTPase Antibody (1C9) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DUT (AAH33645 68 a.a. - 164 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TDIQIALPSGCYGRVAPRSGLAAKHFIDVGAGVIDEDYRGNVGVVLFNFGKEKFEVKKGDRIAQLICERIFYPEIEEVQALDDTERGSGGFGSTGKN
• Specificity: DUT - dUTP pyrophosphatase
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DUT
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IHC-P, IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for dUTPase Antibody (1C9)

• deoxyuridine triphosphatase
• dUTP pyrophosphatasedUTP nucleotidohydrolase
• dUTPasedeoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
• EC 3.6.1.23
• FLJ20622

Background

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for dUTPase Antibody (H00001854-M01)(1)

Publication using H00001854-M01

• Kiyonari S, Iimori M, Matsuoka K et al. The 1,2-Diaminocyclohexane Carrier Ligand in Oxaliplatin Induces p53-Dependent Transcriptional Repression of Factors Involved in Thymidylate Biosynthesis. Mol Cancer Ther. 2015-07-24 [PMID: 26208523]

Bioinformatics

• Gene Symbol: DUT
• Entrez:
  • 1854(Human)
• OMIM:
  • 601266(Human)
• Uniprot:
  • AAH33645(Human)