DUSP4 Antibody (1D12) - Azide and BSA Free

Western Blot: DUSP4 Antibody (1D12) [H00001846-M08] - Analysis of DUSP4 expression in transfected 293T cell line by DUSP4 monoclonal antibody (M08), clone 1D12. Lane 1: DUSP4 transfected lysatE (43 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: DUSP4 Antibody (1D12) [H00001846-M08] - Analysis of monoclonal antibody to DUSP4 on HeLa cell. Antibody concentration 10 ug/ml

ELISA: DUSP4 Antibody (1D12) [H00001846-M08] - Detection limit for recombinant GST tagged DUSP4 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DUSP4 Antibody (1D12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DUSP4 (AAH02671, 1 a.a. ~ 394 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHGTLGLPSGGKCLLLDCRPFLAHSAGYILGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSATEPLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCAAEAASPSGPLRERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLHSPITTSPSC
• Specificity: DUSP4 (1D12)
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DUSP4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DUSP4 Antibody (1D12) - Azide and BSA Free

• dual specificity phosphatase 4
• Dual specificity protein phosphatase hVH2
• EC 3.1.3.16
• EC 3.1.3.48
• HVH2serine/threonine specific protein phosphatase
• MAP kinase phosphatase 2
• Mitogen-activated protein kinase phosphatase 2
• MKP-2dual specificity protein phosphatase 4
• MKP2VH1 homologous phosphatase 2
• TYP
• VH2

Background

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DUSP4
• Entrez:
  • 1846(Human)
• OMIM:
  • 602747(Human)
• Uniprot:
  • AAH02671(Human)