DTX3 Antibody

Western Blot: DTX3 Antibody [NBP1-46127] - (0.3ug/ml) Analysis of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Hu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DTX3 Antibody Summary

• Immunogen: Peptide with sequence C-KNKVTVSKPVWD corresponding to internal region (near N-Terminus) according to NP_848597.1.
• Epitope: internal region (near N Terminus)
• Predicted Species: Human (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DTX3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.2 - 0.5 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in mouse Fetal brain lysates and in NIH3T3 lysates is reported in scientific literature (PMID: 12670957).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DTX3 Antibody

• deltex 3 homolog (Drosophila)
• deltex homolog 3 (Drosophila)
• deltex3
• FLJ34766
• MGC138863
• MGC138864
• protein deltex-3
• RING finger protein 154
• RNF154deltex 3 homolog

Background

DTX3 encodes a probable E3 ubiquitin-protein ligase DTX3 that exists in two isoforms: Isoform 1 id 347 amino acids long at nearly 38 kDA, while isoform 2 is 350 amino acids long at 38 kDA as well. DTX3 is involved in the notch signaling pathway and protein modification and is known to interact with genes UBE2D2, UBE2D3, UBE2L6, UBE2V1, and UBE2W. It is critical in cell to cell communication that affects cell-fate determinations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DTX3 Antibody (NBP1-46127)(2)

Publications using NBP1-46127

• Liu J, Li H, Mao A et al. DCAF13 promotes triple-negative breast cancer metastasis by mediating DTX3 mRNA degradation Cell cycle (Georgetown, Tex.) 2020-12-01 [PMID: 33300431]
• Takeyama K, Aguiar RC, Gu L et al. The BAL-binding protein BBAP and related Deltex family members exhibit ubiquitin-protein isopeptide ligase activity. J Biol Chem 2003-06-01 [PMID: 12670957]

Bioinformatics

• Gene Symbol: DTX3
• Entrez:
  • 196403(Human)
  • 80904(Mouse)
  • 500847(Rat)
• Uniprot:
  • NP_848597.1(Human)