Drebrin 1 Antibody - BSA Free

Immunohistochemistry;Drebrin 1 Antibody[NBP3-30417]Detection of human Drebrin by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Drebrin used at a dilution of 1:1,000 . Detection: DAB

Immunohistochemistry;Drebrin 1 Antibody[NBP3-30427]Detection of mouse Drebrin by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Drebrin used at a dilution of 1:1,000 . Detection: DAB

Immunoprecipitation:Drebrin 1 AntibodyNBP3-30427] -Detection of human Drebrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Drebrin antibody NBP3-30427 (lot NBP3-30427-1) used for IP at 6 µg per reaction. Drebrin was also immunoprecipitated by rabbit anti-Drebrin antibody BL17077. For blotting immunoprecipitated Drebrin, NBP3-30427 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Drebrin 1 Antibody - BSA Free Summary

• Immunogen: Between 599 and 649
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DBN1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Drebrin 1 Antibody - BSA Free

• D0S117E
• DBN1
• Developmentally-regulated brain protein
• DKFZp434D064
• Drba
• Drebrin 1
• Drebrin A
• Drebrin E
• Drebrin E2
• drebrin
• drebrin-1

Background

Drebrins are actin-binding proteins, that are developmentally regulated in the process of neuronal growth. Three isoforms, E1 and E2 (embryonic) and A (adult) are generated by alternative RNA splicing from a single debrin gene in the chicken brain. The expression pattern of each isoform is regulated spatially and temporally in the developing brain. It has been suggested that debrin is one of the intracellular regulators of the neuronal morphogenesis (4). Debrin has also been detected in other kinds of non-neuronal human and bovine tissue but its exact role is unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DBN1