DRAK1 Antibody

Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DRAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DRAK1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DRAK1 Antibody Summary

• Immunogen: E. coli-derived recombinant human DRAK1
  Ala115-Glu313
  Accession # Q9UEE5
• Specificity: Detects human DRAK1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human DRAK2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STK17A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DRAK1 Antibody

• DAP kinase-related apoptosis-inducing protein kinase 1
• DRAK1
• DRAK1death-associated protein kinase-related 1
• EC 2.7.11.1
• serine/threonine kinase 17a (apoptosis-inducing)
• serine/threonine kinase 17a
• serine/threonine-protein kinase 17A
• STK17A

Background

DRAK1 (DAP kinase-related apoptosis-inducing protein kinase 1; also STK17A) is a 55 kDa member of the DAP kinase subfamily, CAMK Ser/Thr protein kinase family of enzymes. It is expressed in osteoclasts, resides in the nucleus and initiates apoptosis. Human DRAK1 is 414 amino acids (aa) in length. It contains an N‑terminal polyPro segment (aa 33‑37) followed by a Ser/Thr kinase domain (aa 64‑321) with a catalytic loop (aa 184‑193). Apoptosis apparently requires an intact kinase domain. No rodent DRAK1 has been reported. Over aa 115‑313, human DRAK1 is 95% aa identical to canine DRAK1, and 65% aa identical to human DRAK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STK17A
• Uniprot:
  • Q9UEE5()