DR1 Antibody

Western Blot: DR1 Antibody [H00001810-B01P] - Analysis of DR1 expression in NIH/3T3.

Immunocytochemistry/Immunofluorescence: DR1 Antibody [H00001810-B01P] - Analysis of purified antibody to DR1 on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: DR1 Antibody [H00001810-B01P] - Analysis of DR1 expression in PC-12.

Western Blot: DR1 Antibody [H00001810-B01P] - Analysis of DR1 expression in Hela S3 NE.

Western Blot: DR1 Antibody [H00001810-B01P] - Analysis of DR1 expression in Raw 264.7.

Western Blot: DR1 Antibody [H00001810-B01P] - Analysis of DR1 expression in Jurkat.

Western Blot: DR1 Antibody [H00001810-B01P] - Analysis of DR1 expression in transfected 293T cell line by DR1 polyclonal antibody. Lane 1: DR1 transfected lysate(19.36 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

DR1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: DR1 (NP_001929.1, 1 a.a. - 176 a.a.) full-length human protein. MASSSGNDDDLTIPRAAINKMIKETLPNVRVANDARELVVNCCTEFIHLISSEANEICNKSEKKTISPEHVIQALESLGFGSYISEVKEVLQECKTVALKRRKASSRLENLGIPEEELLRQQQELFAKARQQQAELAQQEWLQMQQAAQQAQLAAASASASNQAGSSQDEEDDDDI
• Specificity: DR1 - down-regulator of transcription 1, TBP-binding (negative cofactor 2),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: DR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It is also useful for IF.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DR1 Antibody

• Down-regulator of transcription 1
• down-regulator of transcription 1, TBP-binding (negative cofactor 2)
• NC2
• NC2-BETA
• Negative cofactor 2-beta
• protein Dr1
• TATA-binding protein-associated phosphoprotein

Background

This gene encodes a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DR1
• Entrez:
  • 1810(Human)
• Uniprot:
  • NP_001929.1(Human)