DPM2 Antibody

• Reactivity: Mu
• Applications: ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DPM2 Antibody Summary

• Description: Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.
• Immunogen: Peptide with sequence C-YVMLKSQKITKKAQ corresponding to C-Terminus according to NP_034203.1.
• Epitope: C terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DPM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Preliminary experiments gave an approx. 25 kDa band in mouse eye lysates after 0.02 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 9.4 kDa band according to NP_034203.1. The 25 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DPM2 Antibody

• dolichol phosphate-mannose biosynthesis regulatory protein
• dolichol phosphate-mannose synthase 2
• dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit
• FLJ80013
• MGC111193
• MGC21559

Background

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a hydrophobic protein that contains 2 predicted transmembrane domains and a putative ER localization signal near the C terminus. This protein associates with DPM1 in vivo and is required for the ER localization and stable expression of DPM1 and also enhances the binding of dolichol-phosphate to DPM1. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DPM2 Antibody (NBP1-28470)(1)

Publication using NBP1-28470

• Watanabe R, Murakami Y, Marmor MD et al. Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2. EMBO J 2000-08-15 [PMID: 10944123]

Bioinformatics

• Gene Symbol: DPM2
• Entrez:
  • 13481(Mouse)
• Uniprot:
  • NP_034203.1(Human)