DPH2 Antibody - BSA Free

Western Blot: DPH2 Antibody [NBP2-16218] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: DPH2 Antibody [NBP2-16218] - Immunohistochemical analysis of paraffin-embedded Breast carcinoma, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DPH2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human DPH2. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DPH2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DPH2 Antibody - BSA Free

• Diphthamide biosynthesis protein 2 homolog-like 2
• diphthamide biosynthesis protein 2
• diptheria toxin resistance protein required for diphthamide biosynthesis-like 2
• diptheria toxin resistance protein required for diphthamide biosynthesis-like 2(S. cerevisiae)
• DPH2 homolog (S. cerevisiae)
• DPH2 homolog
• DPH2L2diphthamide biosynthesis-like protein 2
• DPH2-like 2 (S. cerevisiae)
• DPH2-like 2
• hsDph2

Background

This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DPH2
• Uniprot:
  • Q9BQC3(Human)