DPH2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: DPH2 Antibody [NBP2-13933] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.
Immunohistochemistry-Paraffin: DPH2 Antibody [NBP2-13933] - Staining of human testis shows strong nuclear positivity in Leydig cells and subset of cells in seminiferous ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DPH2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEP MPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRL
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DPH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DPH2 Protein (NBP2-13933PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Rat (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DPH2 Antibody

  • Diphthamide biosynthesis protein 2 homolog-like 2
  • diphthamide biosynthesis protein 2
  • diptheria toxin resistance protein required for diphthamide biosynthesis-like 2
  • diptheria toxin resistance protein required for diphthamide biosynthesis-like 2(S. cerevisiae)
  • DPH2 homolog (S. cerevisiae)
  • DPH2 homolog
  • DPH2L2diphthamide biosynthesis-like protein 2
  • DPH2-like 2 (S. cerevisiae)
  • DPH2-like 2
  • hsDph2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DPH2