DPH2 Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: DPH2 Antibody [NBP2-13933] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.
Immunohistochemistry-Paraffin: DPH2 Antibody [NBP2-13933] - Staining of human testis shows strong nuclear positivity in Leydig cells and subset of cells in seminiferous ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

DPH2 Antibody - BSA Free Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: DPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DPH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DPH2 Protein (NBP2-13933PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DPH2 Antibody - BSA Free

  • Diphthamide biosynthesis protein 2 homolog-like 2
  • diphthamide biosynthesis protein 2
  • diptheria toxin resistance protein required for diphthamide biosynthesis-like 2
  • diptheria toxin resistance protein required for diphthamide biosynthesis-like 2(S. cerevisiae)
  • DPH2 homolog (S. cerevisiae)
  • DPH2 homolog
  • DPH2L2diphthamide biosynthesis-like protein 2
  • DPH2-like 2 (S. cerevisiae)
  • DPH2-like 2
  • hsDph2

Background

DPH2 is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DPH2