DPH2 Antibody (OTI4E7) - Azide and BSA Free

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Western Blot: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPH2 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human prostate tissue using anti-DPH2 mouse monoclonal antibody.
Flow Cytometry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-DPH2 ...read more
Western Blot: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Analysis of extracts (35ug) from 9 different cell lines by using anti-DPH2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: ...read more
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human colon tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human endometrium tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human liver tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human pancreas tissue using anti-DPH2 mouse monoclonal antibody.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human tonsil using anti-DPH2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI4E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

DPH2 Antibody (OTI4E7) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human DPH2(NP_001375) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DPH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • CyTOF-ready
Theoretical MW
51.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for DPH2 Antibody (OTI4E7) - Azide and BSA Free

  • Diphthamide biosynthesis protein 2 homolog-like 2
  • diphthamide biosynthesis protein 2
  • diptheria toxin resistance protein required for diphthamide biosynthesis-like 2
  • diptheria toxin resistance protein required for diphthamide biosynthesis-like 2(S. cerevisiae)
  • DPH2 homolog (S. cerevisiae)
  • DPH2 homolog
  • DPH2L2diphthamide biosynthesis-like protein 2
  • DPH2-like 2 (S. cerevisiae)
  • DPH2-like 2
  • hsDph2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70585AF350
Alexa Fluor 405 NBP2-70585AF405
Alexa Fluor 488 NBP2-70585AF488
Alexa Fluor 532 NBP2-70585AF532
Alexa Fluor 594 NBP2-70585AF594
Alexa Fluor 647 NBP2-70585AF647
Alexa Fluor 700 NBP2-70585AF700
Alexa Fluor 750 NBP2-70585AF750
Allophycocyanin NBP2-70585APC
Allophycocyanin/Cy7 NBP2-70585APCCY7
Biotin NBP2-70585B
DyLight 350 NBP2-70585UV
DyLight 405 NBP2-70585V
DyLight 488 NBP2-70585G
DyLight 550 NBP2-70585R
DyLight 594 NBP2-70585DL594
DyLight 650 NBP2-70585C
DyLight 680 NBP2-70585FR
DyLight 755 NBP2-70585IR
FITC NBP2-70585F
HRP NBP2-70585H
Janelia Fluor 549 NBP2-70585JF549
Janelia Fluor 646 NBP2-70585JF646
PE NBP2-70585PE
PE/Atto594 NBP2-70585PEATT594
PE/Cy5.5 NBP2-70585PECY55
PE/Cy7 NBP2-70585PECY7
PerCP NBP2-70585PCP

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Bioinformatics

Gene Symbol DPH2