DPH2 Antibody (OTI4E7) - Azide and BSA Free

Western Blot: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPH2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPH2.

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human prostate tissue using anti-DPH2 mouse monoclonal antibody.

Flow Cytometry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-DPH2 antibody, and then analyzed by flow cytometry.

Western Blot: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Analysis of extracts (35ug) from 9 different cell lines by using anti-DPH2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DPH2 mouse monoclonal antibody.

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DPH2 mouse monoclonal antibody.

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DPH2 mouse monoclonal antibody.

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DPH2 mouse monoclonal antibody.

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human colon tissue using anti-DPH2 mouse monoclonal antibody.

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human endometrium tissue using anti-DPH2 mouse monoclonal antibody.

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human liver tissue using anti-DPH2 mouse monoclonal antibody.

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human pancreas tissue using anti-DPH2 mouse monoclonal antibody.

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human tonsil using anti-DPH2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI4E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

DPH2 Antibody (OTI4E7) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human DPH2(NP_001375) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DPH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 51.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for DPH2 Antibody (OTI4E7) - Azide and BSA Free

• Diphthamide biosynthesis protein 2 homolog-like 2
• diphthamide biosynthesis protein 2
• diptheria toxin resistance protein required for diphthamide biosynthesis-like 2
• diptheria toxin resistance protein required for diphthamide biosynthesis-like 2(S. cerevisiae)
• DPH2 homolog (S. cerevisiae)
• DPH2 homolog
• DPH2L2diphthamide biosynthesis-like protein 2
• DPH2-like 2 (S. cerevisiae)
• DPH2-like 2
• hsDph2

Background

DPH2 is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPH2