DPH2 Antibody (OTI4E7) [DyLight 650]

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, IHC
• Clone: OTI4E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

DPH2 Antibody (OTI4E7) [DyLight 650] Summary

• Immunogen: Full length recombinant protein of human DPH2 (NP_001375) produced in HEK293T cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DPH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for DPH2 Antibody (OTI4E7) [DyLight 650]

• Diphthamide biosynthesis protein 2 homolog-like 2
• diphthamide biosynthesis protein 2
• diptheria toxin resistance protein required for diphthamide biosynthesis-like 2
• diptheria toxin resistance protein required for diphthamide biosynthesis-like 2(S. cerevisiae)
• DPH2 homolog (S. cerevisiae)
• DPH2 homolog
• DPH2L2diphthamide biosynthesis-like protein 2
• DPH2-like 2 (S. cerevisiae)
• DPH2-like 2
• hsDph2

Background

DPH2 is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPH2