Human Dopamine beta-Hydroxylase ELISA Kit (Colorimetric)

Human Dopamine beta-Hydroxylase ELISA Kit (Colorimetric) - Standard Curve Reference

Product Discontinued

Human Dopamine beta-Hydroxylase ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Dopamine beta-Hydroxylase in samples. No significant cross-reactivity or interference between Human Dopamine beta-Hydroxylase and analogues was observed.
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.35%
• Intra-Assay: CV% < 4.96%
• Spike Recovery: 90-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: DBH

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Dopamine beta-Hydroxylase ELISA Kit (Colorimetric)

• DBH
• DBM
• dopamine beta-hydroxylase (dopamine beta-monooxygenase)
• Dopamine betaHydroxylase
• Dopamine beta-Hydroxylase
• Dopamine beta-monooxygenase
• DOPBHY
• EC 1.14.17.1

Background

Dopamine beta-hydroxylase belongs to the copper type II, ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of dopamine to noradrenaline. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons being expressed in noradrenergic and adrenergic neurons. DBH serves as a marker of noradrenergic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Dopamine beta-Hydroxylase ELISA Kit (NBP2-67945)(1)

Publication using NBP2-67945

• Ozuna H, Uriarte SM, Demuth DR The Hunger Games: Aggregatibacter actinomycetemcomitans Exploits Human Neutrophils As an Epinephrine Source for Survival Frontiers in immunology 2021-08-12 [PMID: 34456916] (ELISA)

FAQs for Dopamine beta-Hydroxylase ELISA Kit (NBP2-67945). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: DBH