Dopamine beta-Hydroxylase Antibody (DBH41)

Product Discontinued

Dopamine beta-Hydroxylase Antibody (DBH41) Summary

• Immunogen: Full length for dopamine beta-hydrozylase protein was used as the immunogen for this antibody.
• Specificity: This antibody recognizes This antibody specifically recognizes two bands of molecular weight 70 and 75 kDa, corresponding to the soluble and membrane bound forms of the enzyme, respectively. Due to the high sensitivity of the DBH antibody, the presence of the enzyme can be immunologically detected even in areas where only scattered DBH-containing fibers are present. DBH antibody specifically stains DBH-containing neurons and fibers in the rat central nervous system (CNS).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DBH
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry 1:1000
  • Immunohistochemistry 1:1000
  • Western Blot

Reactivity Notes

It exhibits no cross-reactivity with either mouse, Human, rabbit, guinea pig, cat or bovine DBH.

Packaging, Storage & Formulations

• Storage: Lyophilized antibody can be kept at 4C for up to 3 months and should be kept at -20C for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20C) or for long-term storage (-80C). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
• Buffer: PBS, pH7.4
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with 0.1 ml water.

Alternate Names for Dopamine beta-Hydroxylase Antibody (DBH41)

• DBH
• DBM
• dopamine beta-hydroxylase (dopamine beta-monooxygenase)
• Dopamine betaHydroxylase
• Dopamine beta-Hydroxylase
• Dopamine beta-monooxygenase
• DOPBHY
• EC 1.14.17.1

Background

Dopamine beta-hydroxylase belongs to the copper type II, ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of dopamine to noradrenaline. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons being expressed in noradrenergic and adrenergic neurons. DBH serves as a marker of noradrenergic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DBH
• Uniprot:
  • Q05754(Rat)