DOCK4 Antibody (1B3)

Western Blot: DOCK4 Antibody (1B3) [H00009732-M03] - DOCK4 monoclonal antibody (M03), clone 1B3. Western Blot analysis of DOCK4 expression in PC-12 ( Cat # L012V1 ).

ELISA: DOCK4 Antibody (1B3) [H00009732-M03] - Detection limit for recombinant GST tagged DOCK4 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DOCK4 Antibody (1B3) Summary

• Immunogen: DOCK4 (NP_055520, 1867 a.a. ~ 1966 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NQVNEQSAPLPVPVPVPVPSYGGEEPVRKESKTPPPYSVYERTLRRPVPLPHSLSIPVTSEPPALPPKPLAARSSHLENGARRTDPGPRPRPLPRKVSQL
• Specificity: DOCK4 - dedicator of cytokinesis 4 (1B3)
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DOCK4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DOCK4 Antibody (1B3)

• dedicator of cytokinesis 4
• dedicator of cytokinesis protein 4
• KIAA0716FLJ34238
• MGC134911
• MGC134912

Background

This gene is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain, a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated, cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described, but only one has been fully characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DOCK4
• Entrez:
  • 9732(Human)
• Uniprot:
  • NP_055520(Human)