Recombinant Human DNAJC15 His Protein

SDS-Page: DNAJC15 Protein [NBP2-23006]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human DNAJC15 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 58-150 of Human DNAJC15
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSFRIWKPL EQVITETAKK ISTPSFSSYY KGGFEQKMSR REAGLILGVS PSAGKAKIRT AHRRVMILNH PDKGGSPYVA AKINEAKDLL ETTTKH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DNAJC15
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 12.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human DNAJC15 His Protein

• Cell growth-inhibiting gene 22 protein
• DnaJ (Hsp40) homolog, subfamily C, member 15
• DnaJ (Hsp40) homolog, subfamily D, member 1
• DNAJ domain-containing
• dnaJ homolog subfamily C member 15
• DNAJD1
• MCJHSD18
• Methylation-controlled J protein

Background

DNAJC15 contains 1 J domain. This protein is expressed ubiquitously and located on the membrane. DNAJC15 is absent or down-regulated in many advanced cases of ovarian adenocarcinoma, due to hypermethylation and allelic loss. Loss of expression correlates with increased resistance to antineoplastic drugs, such as cisplatin. Recombinant human DNAJC15 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DNAJC15