DNAJA2 Antibody (2A11-F2) - Azide and BSA Free

Western Blot: DNAJA2 Antibody (2A11-F2) [H00010294-M01] - DNAJA2 monoclonal antibody (M01), clone 2A11-F2 Analysis of DNAJA2 expression in A-431.

Immunocytochemistry/Immunofluorescence: DNAJA2 Antibody (2A11-F2) [H00010294-M01] - Analysis of monoclonal antibody to DNAJA2 on HeLa cell. Antibody concentration 10 ug/ml.

ELISA: DNAJA2 Antibody (2A11-F2) [H00010294-M01] - Detection limit for recombinant GST tagged DNAJA2 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 2A11-F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DNAJA2 Antibody (2A11-F2) - Azide and BSA Free Summary

• Immunogen: DNAJA2 (AAH13044, 1 a.a. ~ 412 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANVADTKLYDILGVPPGASENELKKAYRKLAKEYHPDKNPNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNGEGEVINEKDRCKKCEGKKVIKEVKILEVHVDKGMKHGQRITFTGEADQAPGVEPGDIVLLLQEKEHEVFQRDGNDLHMTYKIGLVEALCGFQFTFKHLDGRQIVVKYPPGKVIEPGCVRVVRGEGMPQYRNPFEKGDLYIKFDVQFPENNWINPDRLSELEDLLPSRPEVPNIIGETEEVELQEFDSTRGSGGGQRREAYNDSSDEESSSHHGPGVQCAHQ
• Specificity: DNAJA2 - DnaJ (Hsp40) homolog, subfamily A, member 2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DNAJA2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DNAJA2 Antibody (2A11-F2) - Azide and BSA Free

• cell cycle progression 3 protein
• Cell cycle progression restoration gene 3 protein
• CPR3PRO3015
• Dj3
• DJA2
• DnaJ (Hsp40) homolog, subfamily A, member 2
• dnaJ homolog subfamily A member 2
• DNAJ
• DNJ3
• HIRA interacting protein 4
• HIRA-interacting protein 4
• HIRIP4RDJ2
• Renal carcinoma antigen NY-REN-14

Background

The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNAJA2 Antibody (H00010294-M01)(3)

Publications using H00010294-M01

• Carnini A, Scott LO, Ahrendt E et al. Cell line specific modulation of extracellular a?42 by hsp40. PLoS One 2012-05-29 [PMID: 22666389]
• Xu F, Proft J, Gibbs S et al. Quercetin targets cysteine string protein (CSPalpha) and impairs synaptic transmission. PLoS One 5(6). 2010-06-10 [PMID: 20548785]
• Rosales-Hernez A, Beck KE, Zhao X et al. RDJ2 (DNAJA2) chaperones neural G protein signaling pathways. Cell Stress Chaperones;14(1):71-82. 2009-01-01 [PMID: 18595009]

Bioinformatics

• Gene Symbol: DNAJA2
• Entrez:
  • 10294(Human)
• Uniprot:
  • AAH13044(Human)