DNA Polymerase lambda Antibody

Western Blot: DNA Polymerase lambda Antibody [NB100-81664] - Detection of Human Pol Lambda by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody NB100-81664 used for WB at 0.1 ug/ml. Detection : Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81664] - Detection of human Polymerase lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody NB100-81664 used for IP at 6 ug per reaction. Pol Lambda was also immunoprecipitated by rabbit anti-Pol Lambda antibody NB100-81665. For blotting immunoprecipitated Pol Lambda, NB100-81664 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DNA Polymerase lambda Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human polymerase (DNA directed), lambda using the numbering given in entry NP_037406.1 (GeneID 27343).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  DNA Polymerase lambda Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DNA Polymerase lambda Antibody

• BETAN
• DNA polymerase beta-2
• DNA polymerase beta-N
• DNA polymerase kappa
• DNA polymerase lambda
• EC 2.7.7.7
• EC 4.2.99.-
• FLJ46002
• Pol beta2
• Pol Lambda
• POLKAPPA
• polymerase (DNA directed), lambda

Background

DNA polymerase lambda (pol lambda) is a DNA polymerase involved in base excision repair (BER). Pol lambda possesses terminal transferase and 5'-deoxyribose-5-phosphate lyase activity. These activities have been found to be coordinated by PCNA and replication protein A. Alternate names for pol lambda include DNA polymerase kappa, DNA polymerase beta-2, pol beta-2, POLL, POLL-KAPPA, and BETA-N.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA Polymerase lambda Antibody (NB100-81664)(1)

Publication using NB100-81664

• Cerqueira PG, Meyer D, Zhang L et al. Saccharomyces cerevisiae DNA polymerase IV overcomes Rad51 inhibition of DNA polymerase ? in Rad52-mediated direct-repeat recombination Nucleic acids research 2023-04-18 [PMID: 37070185]

Bioinformatics

• Gene Symbol: POLL
• Entrez:
  • 27343(Human)
• Uniprot:
  • Q9UGP5(Human)