DNA Polymerase epsilon subunit 3 Antibody - BSA Free

Western Blot: DNA Polymerase epsilon subunit 3 Antibody [NBP2-92951] - Western blot analysis of extracts from normal (control) and [KO Validated] DNA Polymerase epsilon subunit 3 Rabbit pAb knockout (KO) 293T cells, using [KO Validated] DNA Polymerase epsilon subunit 3 Rabbit pAb antibody (NBP2-92951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western Blot: DNA Polymerase epsilon subunit 3 Antibody [NBP2-92951] - Analysis of extracts of various cell lines, using DNA Polymerase epsilon subunit 3 . Exposure time: 90s.

• Reactivity: Hu, Mu
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DNA Polymerase epsilon subunit 3 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human DNA Polymerase epsilon subunit 3 (NP_059139.3). MAERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTLNASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSEEQDKSRDEDNDEDEERLEEEEQNEEEEVDN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLE3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DNA Polymerase epsilon subunit 3 Antibody - BSA Free

• Arsenic-transactivated protein
• asTP
• CHARAC17arsenic transactivated protein
• CHRAC-17
• CHRAC17Ybl1
• Chromatin accessibility complex 17 kDa protein
• DNA polymerase epsilon p17 subunit
• DNA polymerase epsilon subunit 3
• DNA polymerase epsilon subunit p17
• DNA polymerase II subunit 3
• EC 2.7.7.7
• histone fold protein CHRAC17
• huCHRAC17
• p17
• polymerase (DNA directed), epsilon 3 (p17 subunit)
• YBL1

Background

DNA polymerase epsilon (POLE) is an enzyme that helps catalyze in the polymerization of deoxyribonucleotides into a DNA strand. The C-terminus of DNA polymerase E subunit complexes with subunits B and C, whereas the N-terminal domain contains an active polymerase domain.

DNA Pol E antibodies are useful for DNA replication and repair studies and in research on mitosis and cell replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLE3