DLL4 Antibody

Western Blot: DLL4 Antibody [NBP2-89151] - Analysis of DLL4 on human lung tissue lysate using anti-DLL4 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: DLL4 Antibody [NBP2-89151] - Staining DLL4 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: DLL4 Antibody [NBP2-89151] - Analysis of paraffin-embedded human kidney tissue using anti-DLL4 antibody. Counter stained with hematoxylin.

Flow Cytometry: DLL4 Antibody [NBP2-89151] - Analysis of 293T cells with DLL4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: DLL4 Antibody [NBP2-89151] - Staining DLL4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: DLL4 Antibody [NBP2-89151] - Staining DLL4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

DLL4 Antibody Summary

• Immunogen: Synthetic peptide within human DLL4 aa 121-159. (SwissProt: Q9NR61 Human; SwissProt: D3ZHH1 Rat)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DLL4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DLL4 Antibody

• Delta 4 precursor
• delta 4
• delta ligand 4
• delta4
• delta-like 4 (Drosophila)
• delta-like 4 homolog (Drosophila)
• delta-like 4 homolog
• delta-like 4 protein
• delta-like protein 4
• DLL4
• Drosophila Delta homolog 4
• hdelta2
• MGC126344
• notch ligand delta-2
• notch ligand DLL4

Background

DELTA-4 is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. DELTA-4 plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLL4