DLL1 Antibody (251127) [Unconjugated]

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 251127
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DLL1 Antibody (251127) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DLL1
  Ser22-Gly540
  Accession # AAG09716
• Specificity: Detects recombinant human DLL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse DLL4 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DLL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human DLL1 Antibody (Catalog # MAB1818)
  ELISA Detection: Human DLL1 Biotinylated Antibody (Catalog # BAF1818)
  Standard: Recombinant Human DLL1 (Catalog # 1818-DL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DLL1 Antibody (251127) [Unconjugated]

• delta (Drosophila)-like 1
• Delta 1
• Delta
• Delta1
• delta-like 1 (Drosophila)
• delta-like protein 1
• DL1
• DLL1
• Drosophila Delta homolog 1
• H-Delta-1

Background

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. 
2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. 
3. Karanu, F.N. et al. (2001) Blood 97:1960. 
4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 
5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. 
6. Takahashi, Y. et al. (2003) Development 130:4259. 
7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
8. Kiernan, A.E. et al. (2005) Development 132:4353.
9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.
12. Limbourg, A. et al. (2007) Circ. Res. 100:363.
13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DLL1 Antibody (MAB1818)(3)

Publications using MAB1818

• Yuan Li, Yuekun Zhang, Xiaosa Liu, Mingchen Wang, Peng Wang, Jianbo Yang et al. Lutein inhibits proliferation, invasion and migration of hypoxic breast cancer cells via downregulation of HES1 International Journal of Oncology 2018-03-23 [PMID: 29620169]
• Le Friec G, Sheppard D, Whiteman P, Karsten C, Shamoun S, Laing A, Bugeon L, Dallman M, Melchionna T, Chillakuri C, Smith R, Drouet C, Couzi L, Fremeaux-Bacchi V, Kohl J, Waddington S, McDonnell J, Baker A, Handford P, Lea S, Kemper C The CD46-Jagged1 interaction is critical for human TH1 immunity. Nat Immunol, 2012-10-21;13(12):1213-21. 2012-10-21 [PMID: 23086448] (Flow Cytometry, Human)
• Uenotsuchi T, Takeuchi S, Matsuda T, Urabe K, Koga T, Uchi H, Fukagawa S, Kawasaki M, Kajiwara H, Moroi Y, Furue M Differential induction of Th1-prone immunity by human dendritic cells activated with Sporothrix schenckii of cutaneous and visceral origins to determine their different virulence. Int. Immunol., 2006-10-11;18(12):1637-46. 2006-10-11 [PMID: 17035348] (Western Blot, Human)

Bioinformatics

• Gene Symbol: DLL1
• Uniprot:
  • AAG09716()